Phenotypes associated with this allele

• Allele Symbol: BC004004^em1Nju
• Allele Name: endonuclease-mediated mutation 1, Model Animal Research Center of Nanjing University
• Allele ID: MGI:7336199
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	BC004004^em1Nju/BC004004^em1Nju	C57BL/6J-BC004004^em1Nju	MGI:7336283

Genotype
MGI:7336283

hm1

• Allelic Composition: BC004004^em1Nju/BC004004^em1Nju
• Genetic Background: C57BL/6J-BC004004^em1Nju

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

abnormal autophagosome formation ( J:328326 )

• marker analysis shows increased autophagosome formation in lung fibroblasts

• lung fibroblasts exhibit more autophagosomes than wild-type cells

enhanced autophagy ( J:328326 )

• lung fibroblasts exhibit increased autophagy

• lung fibroblasts show enhanced autophagic activity after starvation

decreased hydroxyproline level ( J:328326 )

• hydroxyproline content of lung fibroblasts does not increase in response to TGF-beta1 stimulation as in control cells

decreased collagen level ( J:328326 )

• lung fibroblasts contain less collagen alpha-(I) chain

abnormal enzyme/coenzyme activity ( J:328326 )

• lung fibroblasts exhibit enhanced cathepsin B activity, indicating enhanced activity of lysosomal cysteine proteases

decreased susceptibility to injury ( J:328326 )

• mice exhibit attenuation of bleomycin-induced pulmonary injury, with mice showing no decrease in body weight, decrease in inflammatory cell infiltration in lungs and destruction of parenchyma, less severe accumulation of total collagen and pulmonary fibrosis, and decreased lung hydroxyproline content

cellular

abnormal lysosome morphology ( J:328326 )

• lung fibroblasts exhibit more lysosomes than wild-type cells

abnormal autophagosome formation ( J:328326 )

• marker analysis shows increased autophagosome formation in lung fibroblasts

• lung fibroblasts exhibit more autophagosomes than wild-type cells

enhanced autophagy ( J:328326 )

• lung fibroblasts exhibit increased autophagy

• lung fibroblasts show enhanced autophagic activity after starvation

abnormal fibroblast physiology ( J:328326 )

• primary lung fibroblasts exhibit decreased collage production and contain less collagen alpha-(I) chain

increased fibroblast apoptosis ( J:328326 )

• increase in the percentage of primary lung fibroblasts undergoing early-phase apoptosis

• after TGF-beta1 stimulation, the percentage of primary lung fibroblasts undergoing early apoptosis is still higher than in wild-type cells

decreased lung fibroblast proliferation ( J:328326 )

• primary lung fibroblasts exhibit decreased cell proliferation after TGF-beta1 stimulation, with a decrease in the percentage of cells in S phase and an increase in the G1 phase