Phenotypes associated with this allele

• Allele Symbol: Mir32^em1Gpt
• Allele Name: endonuclease-mediated mutation 1, GemPharmatech Co., Ltd
• Allele ID: MGI:7302297
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Mir32^em1Gpt/Mir32^em1Gpt Alb^em1(icre)Gpt/Alb^+	C57BL/6JGpt-Mir32^em1Gpt Alb^em1(icre)Gpt	MGI:7580538

Genotype
MGI:7580538

cn1

• Allelic Composition: Mir32^em1Gpt/Mir32^em1Gpt; Alb^em1(icre)Gpt/Alb⁺
• Genetic Background: C57BL/6JGpt-Mir32^em1Gpt Alb^em1(icre)Gpt

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

liver/biliary system

decreased liver cholesterol level ( J:344660 )

• mice fed a high-fat diet (HFD) for 12 weeks exhibit significantly lower hepatic cholesterol levels than diet-matched control mice

• however, hepatic cholesterol levels are relatively normal under standard-fat diet (SFD) conditions

decreased liver triglyceride level ( J:344660 )

• HFD-fed mice exhibit significantly lower hepatic TG levels than diet-matched control mice

• however, hepatic TG levels are normal under SFD conditions

decreased liver weight ( J:344660 )

• HFD-fed mice exhibit a significantly lower liver weight to body weight ratio (LW/BW) than diet-matched controls

• however, LW/BW ratio is normal under SFD conditions

decreased susceptibility to diet-induced hepatic steatosis ( J:344660 )

• mice fed a HFD for 12 weeks show significantly less hepatic steatosis than diet-matched controls, as determined by liver macroscopic appearance, H&E staining, Oil Red O staining, LW/BW ratio, and hepatic lipid contents

• administration of Ad-shRNA targeting INSIG1 (insulin induced gene 1, a direct target of Mir32) abrogates the alleviation of hepatic steatosis, insulin resistance, hyperlipidemia, and overexpression of lipogenic genes in HFD-fed mice

abnormal liver physiology ( J:344660 )

• HFD-fed mice show activation of AKT signaling in the liver while mRNA and protein levels of hepatic genes involved in fatty acyl-CoA, triglyceride, and cholesterol biosynthesis are markedly reduced

• HFD-fed mice show significantly alleviated hepatic endoplasmic reticulum stress relative to diet-matched control mice

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:344660 )

N • mice exhibit normal oxygen consumption, carbon dioxide production, respiratory exchange rate (RER), and heat generation regardless of whether they are fed a HFD or SFD

decreased circulating cholesterol level ( J:344660 )

• HFD-fed mice exhibit significantly lower total serum cholesterol levels than diet-matched control mice

decreased circulating LDL cholesterol level ( J:344660 )

• HFD-fed mice exhibit significantly lower serum LDL cholesterol levels than diet-matched control mice

decreased circulating alanine transaminase level ( J:344660 )

• HFD-fed mice exhibit significantly lower serum ALT levels than diet-matched control mice

• however, serum ALT levels are relatively normal under SFD conditions

decreased circulating aspartate transaminase level ( J:344660 )

• HFD-fed mice exhibit significantly lower serum AST levels than diet-matched control mice

• however, serum AST levels are not significantly altered under SFD conditions

improved glucose tolerance ( J:344660 )

• HFD-fed mice show improved glucose tolerance relative to diet-matched control mice, as indicated by an intraperitoneal glucose tolerance test (IPGTT)

increased insulin sensitivity ( J:344660 )

• HFD-fed mice show enhanced insulin sensitivity relative to diet-matched control mice, as indicated by an insulin tolerance test (IPITT

decreased liver cholesterol level ( J:344660 )

• mice fed a high-fat diet (HFD) for 12 weeks exhibit significantly lower hepatic cholesterol levels than diet-matched control mice

• however, hepatic cholesterol levels are relatively normal under standard-fat diet (SFD) conditions

decreased liver triglyceride level ( J:344660 )

• HFD-fed mice exhibit significantly lower hepatic TG levels than diet-matched control mice

• however, hepatic TG levels are normal under SFD conditions

abnormal lipid metabolism ( J:344660 )

• lipidomic analysis of livers from HFD-fed mice showed a significant reduction in triglyceride and cholesterol ester species relative to diet-matched controls; specifically, TG_48:1, TG_48:2, TG_48:3, TG_49:1, TG_50:1, TG_50:2, TG_50:3, TG_52:1, TG_54:2, CE_22:6, and CE_18:2 are dramatically reduced

cellular

decreased endoplasmic reticulum stress ( J:344660 )

• HFD-fed mice show significantly alleviated hepatic endoplasmic reticulum stress relative to diet-matched control mice

growth/size/body

growth/size/body region phenotype ( J:344660 )

N • mice exhibit normal body weight regardless of whether they are fed a HFD or SFD

behavior/neurological

behavior/neurological phenotype ( J:344660 )

N • mice exhibit normal food intake and total activity regardless of whether they are fed a HFD or SFD