Analysis Tools
cardiovascular system
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• in culture, primary cardiomyocytes isolated from 4 week-old mice show a significant increase in cytosolic calcium levels
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• upon transverse aortic constriction (TAC) surgery, mice exhibit a further reduction of mitochondrial area and a greater increase of mitochondrial structural damage in myocardial tissues than TAC-treated controls
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• under physiological conditions, 12-month-old mice show an increase in LV internal diameter, consistent with an age-dependent decline in cardiac function
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• under physiological conditions, mice show an age-dependent decline in LV function that is pronounced at 12 months of age, as determined by 3-D LV radial strain maps and global longitudinal strain curves
• echocardiographic images from parasternal long- and short-axis views show a significant reduction in ejection fraction (EF) and fractional shortening (FS) at 12 months of age
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• under physiological conditions, 12-month-old mice show more severe LV diastolic dysfunction with a significantly lower mitral valve E/A ratio (early to late diastolic transmitral flow velocity) than controls
• however, diastolic dysfunction is similar to controls after TAC surgery
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• 12-month-old mice subjected to TAC surgery for 4 weeks show exacerbated cardiac dysfunction with a greater reduction in descending aortic blood flow and radial and longitudinal strains, and more severe LV systolic dysfunction than TAC-treated controls as revealed by M-mode echocardiography and measurements of EF and FS
• after TAC, mice exhibit increased heart size, higher heart weight to body weight and heart weight to tibia length ratios, larger cross-sectional areas of cardiomyocytes, more perivascular and interstitial fibrosis, upregulation of hypertrophic genes (including Nppa/Anf and Myh7), and higher protein levels of NPPA/ANF and MYH7/beta-MHC in cardiac tissues than TAC-treated controls
• cardiac-specific overexpression of GPLD1 by intramyocardial injection-mediated delivery of AAV9-GPLD1 attenuates the cardiac dysfunction and hypertrophy and reduces collagen deposition in mice undergoing TAC
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homeostasis/metabolism
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• in culture, primary cardiomyocytes isolated from 4 week-old mice show a significant increase in cytosolic calcium levels
|
|
• 12-month-old mice subjected to TAC surgery for 4 weeks show exacerbated cardiac dysfunction with a greater reduction in descending aortic blood flow and radial and longitudinal strains, and more severe LV systolic dysfunction than TAC-treated controls as revealed by M-mode echocardiography and measurements of EF and FS
• after TAC, mice exhibit increased heart size, higher heart weight to body weight and heart weight to tibia length ratios, larger cross-sectional areas of cardiomyocytes, more perivascular and interstitial fibrosis, upregulation of hypertrophic genes (including Nppa/Anf and Myh7), and higher protein levels of NPPA/ANF and MYH7/beta-MHC in cardiac tissues than TAC-treated controls
• cardiac-specific overexpression of GPLD1 by intramyocardial injection-mediated delivery of AAV9-GPLD1 attenuates the cardiac dysfunction and hypertrophy and reduces collagen deposition in mice undergoing TAC
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• under sham conditions, mice exhibit a significant increase in protein levels of PLAUR (plasminogen activator, urokinase receptor; aka uPAR) in cardiac tissues
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muscle
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• in culture, primary cardiomyocytes isolated from 4 week-old mice show a significant increase in cytosolic calcium levels
|
|
• upon transverse aortic constriction (TAC) surgery, mice exhibit a further reduction of mitochondrial area and a greater increase of mitochondrial structural damage in myocardial tissues than TAC-treated controls
|
|
• under physiological conditions, mice show an age-dependent decline in LV function that is pronounced at 12 months of age, as determined by 3-D LV radial strain maps and global longitudinal strain curves
• echocardiographic images from parasternal long- and short-axis views show a significant reduction in ejection fraction (EF) and fractional shortening (FS) at 12 months of age
|
|
• under physiological conditions, 12-month-old mice show more severe LV diastolic dysfunction with a significantly lower mitral valve E/A ratio (early to late diastolic transmitral flow velocity) than controls
• however, diastolic dysfunction is similar to controls after TAC surgery
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cellular
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• upon transverse aortic constriction (TAC) surgery, mice exhibit a further reduction of mitochondrial area and a greater increase of mitochondrial structural damage in myocardial tissues than TAC-treated controls
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