Phenotypes associated with this allele

• Allele Symbol: Spindoc^em1Mtb
• Allele Name: endonuclease-mediated mutation 1, Mark T Bedford
• Allele ID: MGI:6864335
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Spindoc^em1Mtb/Spindoc^em1Mtb	FVB-Spindoc^em1Mtb	MGI:7481913

Genotype
MGI:7481913

hm1

• Allelic Composition: Spindoc^em1Mtb/Spindoc^em1Mtb
• Genetic Background: FVB-Spindoc^em1Mtb

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

mortality/aging ( J:319478 )

N • heterozygous intercrosses generate viable homozygotes; the birth ratio is consistent with Mendelian inheritance

increased mortality induced by ionizing radiation ( J:319478 )

• when exposed to 6 Gy of IR and maintained for up to 35 days, all mice die within 20 days post IR treatment whereas 15-20% of IR-treated wild-type controls survive to day 35

growth/size/body

decreased body weight ( J:319478 )

• at P21, body weight is 10-20% lower than that in wild-type controls

homeostasis/metabolism

abnormal enzyme/coenzyme activity ( J:319478 )

• thymus extracts from nontreated mice show a dramatic reduction in PARP1-mediated poly-ADP-ribosylation (PARylation) relative to wild-type controls

• after 3 hours recovery from 6Gy IR treatment, PARylation within thymic tissue is significantly lower than that in similarly treated wild-type controls

increased mortality induced by ionizing radiation ( J:319478 )

• when exposed to 6 Gy of IR and maintained for up to 35 days, all mice die within 20 days post IR treatment whereas 15-20% of IR-treated wild-type controls survive to day 35

cellular

increased cellular sensitivity to X-ray irradiation ( J:319478 )

• at 3 hours post ionizing radiation (IR) treatment with 6Gy of X-rays, PARP1 [poly (ADP-ribose) polymerase family, member 1] has undergone complete cleavage in thymic tissue, indicating IR-induced apoptosis

• after 3 hours recovery from 6Gy IR treatment, small intestines show a significantly higher % of cleaved Caspase-3 positive nuclei than similarly treated wild-type intestines