Phenotypes associated with this allele

• Allele Symbol: Lrrc4b^tm1Lex
• Allele Name: targeted mutation 1, Lexicon Genetics
• Allele ID: MGI:6501150
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lrrc4b^tm1Lex/Lrrc4b^tm1Lex	B6.129S-Lrrc4b^tm1Lex	MGI:6502629
hm2	Lrrc4b^tm1Lex/Lrrc4b^tm1Lex	involves: 129S/SvEv * C57BL/6J	MGI:6502631
ht3	Lrrc4b^tm1Lex/Lrrc4b^+	B6.129S-Lrrc4b^tm1Lex	MGI:6502630

Genotype
MGI:6502629

hm1

• Allelic Composition: Lrrc4b^tm1Lex/Lrrc4b^tm1Lex
• Genetic Background: B6.129S-Lrrc4b^tm1Lex

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality, incomplete penetrance ( J:276446 )

• some mice die between P18 and P30

postnatal lethality, incomplete penetrance ( J:276446 )

• some mice die between P18 and P30

preweaning lethality, incomplete penetrance ( J:276446 )

• Background Sensitivity: unlike mice on a mixed background, fewer than expected mice congenic on a C57BL/6 background are present at P7

behavior/neurological

behavior/neurological phenotype ( J:276446 )

N • mice exhibit no autistic-like phenotypes and exhibit normal memory in novel-object recognition

impaired spatial learning ( J:276446 )

• in a Morris water maze during learning, probe, and reversal phases

abnormal eating behavior ( J:276446 )

• weaning is shifted by about a week

• difficulty eating pellets from an overhead grid after weaning

• however, mice eat pellets when food is placed on the cage floor

decreased anxiety-related response ( J:276446 )

• in an elevated plus maze and in a light-dark chamber test

increased vertical activity ( J:276446 )

• but reduced climbing activity

increased locomotor activity ( J:276446 )

• in an open-field test without alteration in thigmotaxis

• however, mice exhibit normal activity in Laboras cages

nervous system

nervous system phenotype ( J:276446 )

N • Background Sensitivity: [BKG] unlike mice on a mixed background, mice on a congenic C57BL/6 background exhibit normal NMDA to AMPA ratio

abnormal brain morphology ( J:276446 )

• at 10 weeks of age

enlarged lateral ventricles ( J:276446 )

enlarged third ventricle ( J:276446 )

reduced long-term potentiation ( J:276446 )

• induced by high-frequency stimulation and almost completely abolished when induced by low-frequency stimulation

growth/size/body

decreased body weight ( J:276446 )

postnatal growth retardation ( J:276446 )

Genotype
MGI:6502631

hm2

• Allelic Composition: Lrrc4b^tm1Lex/Lrrc4b^tm1Lex
• Genetic Background: involves: 129S/SvEv * C57BL/6J

mortality/aging

mortality/aging ( J:276446 )

N • Background Sensitivity: unlike mice on a congenic C57BL/6 background, mice on a mixed background exhibit normal birth rate and survival

behavior/neurological

behavior/neurological phenotype ( J:276446 )

N • mice exhibit no autistic-like phenotypes and exhibit normal memory in novel-object recognition and fear conditioning

increased susceptibility to pharmacologically induced seizures ( J:276446 )

• in response to PTZ

impaired spatial learning ( J:276446 )

• in a Morris water maze during learning, probe, and reversal phases

• in a rotarod test

decreased anxiety-related response ( J:276446 )

• in an elevated plus maze, mice spend more time in the open arms and decreased time in the closed arms compared with wild-type mice

• D-cycloserine does not rescue anxiolytic-like behavior

• however, performance in a light-dark chamber test is normal

decreased thigmotaxis ( J:276446 )

• Background Sensitivity: unlike mice on a congenic C57BL/6 background, mice on a mixed background spend more time in the center of an open-field compared with wild-type mice

impaired coordination ( J:276446 )

• in a rotarod test

hyperactivity ( J:276446 )

• in an open-field test, in the home cage after habituation, and in an elevated plus maze

• however, treatment with D-cycloserine rescues hyperactivity

increased vertical activity ( J:276446 )

• increased rearing but reduced climbing activity

nervous system

nervous system phenotype ( J:276446 )

N • mice exhibit normal synapse development, basal excitatory transmission in the hippocampus, and spontaneous and miniature IPSCs

increased susceptibility to pharmacologically induced seizures ( J:276446 )

• in response to PTZ

decreased brain size ( J:276446 )

• slight

small thalamus ( J:276446 )

abnormal brain wave pattern ( J:276446 )

• decreased alpha oscillations and increased theta oscillations in the temporal, but not frontal lobes

abnormal excitatory postsynaptic currents ( J:276446 )

• Background Sensitivity: unlike mice on a congenic background, mice on a mixed background exhibit decreased ratio of evoked NMDAR to AMPAR-mediated EPSCs compared with wild-type mice

• reduced amplitude and frequency in mice treated with AMPAR blocker 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(F)quinoxaline (NBQX) and under a low-magnesium condition

• however, treatment with D-cycloserine rescues NMDAR function

decreased excitatory postsynaptic current amplitude ( J:276446 )

• in mice treated with AMPAR blocker 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(F)quinoxaline (NBQX) and under a low-magnesium condition

increased excitatory postsynaptic current amplitude ( J:276446 )

• increased frequency and amplitude of spontaneous EPSCs

• however, miniature EPSC frequency and amplitudes are normal

decreased excitatory postsynaptic current frequency ( J:276446 )

• in mice treated with AMPAR blocker 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(F)quinoxaline (NBQX) and under a low-magnesium condition

reduced long-term potentiation ( J:276446 )

• induced by high-frequency stimulation and almost completely abolished when induced by low-frequency stimulation

• however, metabotropic glutamate receptor-induced LTD is normal and inhibition of Akt normalizes the suppressed LTD

growth/size/body

growth/size/body region phenotype ( J:276446 )

N • unlike mice on a congenic C57BL/6 background, mice on a mixed background exhibit normal postnatal growth

decreased body weight ( J:276446 )

• slight

Genotype
MGI:6502630

ht3

• Allelic Composition: Lrrc4b^tm1Lex/Lrrc4b⁺
• Genetic Background: B6.129S-Lrrc4b^tm1Lex

behavior/neurological

decreased anxiety-related response ( J:276446 )

• in the light-dark test

decreased locomotor activity ( J:276446 )

• in the open-field test