Phenotypes associated with this allele

• Allele Symbol: Stag2^tm1.1Alos
• Allele Name: targeted mutation 1.1, Ana Losada
• Allele ID: MGI:6490260
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Ndor1^Tg(UBC-cre/ERT2)1Ejb/Ndor1^+ Stag2^tm1.1Alos/Stag2^tm1.1Alos	involves: 129S6/SvEvTac * C57BL/6NCrl	MGI:6490362
cn2	Ndor1^Tg(UBC-cre/ERT2)1Ejb/Ndor1^+ Stag2^tm1.1Alos/Y	involves: 129S6/SvEvTac * C57BL/6NCrl	MGI:6490363
cn3	Stag2^tm1.1Alos/Y Tg(CAG-cre)1Nagy/0	involves: 129S6/SvEvTac * C57BL/6NCrl	MGI:6490364

Genotype
MGI:6490362

cn1

• Allelic Composition: Ndor1^{Tg(UBC-cre/ERT2)1Ejb}/Ndor1⁺; Stag2^tm1.1Alos/Stag2^tm1.1Alos
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6NCrl

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:298088 )

♀ • 4 weeks after initiation of tamoxifen treatment

cellular

abnormal mitosis ( J:298088 )

♀ • tamoxifen-treated mouse embryonic fibroblasts exhibit increased chromosome adhesion and mis-segregation compared with control cells

increased fibroblast apoptosis ( J:298088 )

♀ • increased caspase-3-positive cells in tamoxifen-treated mouse embryonic fibroblasts

cellular necrosis ( J:298088 )

♀ • 8 week old tamoxifen-treated mice exhibit increased intestinal erosion and necrosis compared with wild-type mice

abnormal enterocyte proliferation ( J:298088 )

♀ • reduced in intestinal crypts of tamoxifen-treated mice indicating reduced cellular renewal

decreased fibroblast proliferation ( J:298088 )

♀ • increased doubling time in tamoxifen-treated mouse embryonic fibroblasts

digestive/alimentary system

abnormal enterocyte proliferation ( J:298088 )

♀ • reduced in intestinal crypts of tamoxifen-treated mice indicating reduced cellular renewal

abnormal intestine morphology ( J:298088 )

♀ • 8 week old tamoxifen-treated mice exhibit increased intestinal erosion and necrosis compared with wild-type mice

growth/size/body

decreased body weight ( J:298088 )

♀ • in tamoxifen-treated mice

hematopoietic system

hematopoietic system phenotype ( J:298088 )

♀ • tamoxifen-treated mice exhibit normal adult hematopoiesis

abnormal hematopoietic stem cell physiology ( J:298088 )

♀ • enhanced self-renewal in cells from tamoxifen-treated mice

neoplasm

neoplasm ( J:298088 )

♀ • tamoxifen-treated mice do not exhibit increased spontaneous tumors

Genotype
MGI:6490363

cn2

• Allelic Composition: Ndor1^{Tg(UBC-cre/ERT2)1Ejb}/Ndor1⁺; Stag2^tm1.1Alos/Y
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6NCrl

mortality/aging

premature death ( J:298088 )

♂ • 4 weeks after initiation of tamoxifen treatment

cellular

abnormal mitosis ( J:298088 )

♂ • tamoxifen-treated mouse embryonic fibroblasts exhibit increased chromosome adhesion and mis-segregation compared with control cells

increased fibroblast apoptosis ( J:298088 )

♂ • increased caspase-3-positive cells in tamoxifen-treated mouse embryonic fibroblasts

cellular necrosis ( J:298088 )

♂ • 8 week old tamoxifen-treated mice exhibit increased intestinal erosion and necrosis compared with wild-type mice

abnormal enterocyte proliferation ( J:298088 )

♂ • reduced in intestinal crypts of tamoxifen-treated mice indicating reduced cellular renewal

decreased fibroblast proliferation ( J:298088 )

♂ • increased doubling time in tamoxifen-treated mouse embryonic fibroblasts

digestive/alimentary system

abnormal enterocyte proliferation ( J:298088 )

♂ • reduced in intestinal crypts of tamoxifen-treated mice indicating reduced cellular renewal

abnormal intestine morphology ( J:298088 )

♂ • 8 week old tamoxifen-treated mice exhibit increased intestinal erosion and necrosis compared with wild-type mice

growth/size/body

decreased body weight ( J:298088 )

♂ • in tamoxifen-treated mice

hematopoietic system

hematopoietic system phenotype ( J:298088 )

♂N • tamoxifen-treated mice exhibit normal adult hematopoiesis

abnormal hematopoietic stem cell physiology ( J:298088 )

♂ • enhanced self-renewal in cells from tamoxifen-treated mice

neoplasm

neoplasm ( J:298088 )

♂N • tamoxifen-treated mice do not exhibit increased spontaneous tumors

Genotype
MGI:6490364

cn3

• Allelic Composition: Stag2^tm1.1Alos/Y; Tg(CAG-cre)1Nagy/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6NCrl

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:298088 )

♂ • mice die between E9.5 and E12.5

cardiovascular system

abnormal cardiac outflow tract development ( J:298088 )

♂ • shortened length and rightward turning at the junction with the ventricular myocardium in mildly affect mice at E9.5

abnormal heart development ( J:298088 )

♂ • distended atria and ventricles at E9.5

♂ • distended OFT and IFT

♂ • severe cardiac anomalies at E10.5 in all mice with extensive necrosis and apoptosis

common atrium ( J:298088 )

♂

decreased heart right ventricle size ( J:298088 )

♂ • in mildly affect mice at E9.5

cellular

decreased cell proliferation ( J:298088 )

♂ • in E9.5 embryos

embryo

embryonic growth retardation ( J:298088 )

♂ • indicated by reduced somite number at E9.5 measuring a day behind

decreased embryo size ( J:298088 )

♂ • mild and severe at E9.5

growth/size/body

embryonic growth retardation ( J:298088 )

♂ • indicated by reduced somite number at E9.5 measuring a day behind

decreased embryo size ( J:298088 )

♂ • mild and severe at E9.5