Phenotypes associated with this allele

• Allele Symbol: Snhg15^tm1Nju
• Allele Name: targeted mutation 1, Model Animal Research Center of Nanjing University
• Allele ID: MGI:6478873
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Snhg15^tm1Nju/Snhg15^tm1Nju	involves: C57BL/6J	MGI:6478904
cx2	Dmd^mdx/Dmd^mdx Snhg15^tm1Nju/Snhg15^tm1Nju	involves: C57BL/6J * C57BL/10ScSn	MGI:6478907

Genotype
MGI:6478904

hm1

• Allelic Composition: Snhg15^tm1Nju/Snhg15^tm1Nju
• Genetic Background: involves: C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

muscle

muscle phenotype ( J:291983 )

N • normal skeletal muscle fiber histology at age P7, age 8 weeks, and in E18.5 embryos

N • normal number of Pax7+ quiescent stem cells (QSCs)

N • normal stem cell apoptosis rates in vitro

delayed skeletal muscle regeneration ( J:291983 )

• delayed muscle regeneration after BaCl₂ injection into tibialis anterior (TA) muscles at age 89 weeks: reduced number of myofibers with centrally located nuclei and reduced number of embryonic myosin heavy chain expressing myofibers 4 days after injection; reduced number of Pax7+ and MyoD+ cells 3 days after injection

• reduced myoblast proliferation after BaCl₂ injection

• reduced in vitro proliferation of stem cells after BaCl₂ injection

• increased fusion ability of stem cells in vitro (increased propensity for differentiation)

• normal regeneration activity 7 days after BaCl₂ injection into tibialis anterior (TA) muscles at age 8-9 weeks, normal full regeneration 28 days after injection and normal number of Pax7+ cells 1 month after injection

cellular

abnormal mitotic spindle morphology ( J:291983 )

• defects in kinetochoremicrotubule attachment and spindle formation resulting in chromosome misalignments in activated stem cells (ASCs) in vitro

immune system

immune system phenotype ( J:291983 )

N • normal spleen histology

liver/biliary system

liver/biliary system phenotype ( J:291983 )

N • normal liver histology

growth/size/body

growth/size/body region phenotype ( J:291983 )

N • normal body size and weight

renal/urinary system

renal/urinary system phenotype ( J:291983 )

N • normal kidney histology

reproductive system

reproductive system phenotype ( J:291983 )

N • fertile

N • normal ovarian histology

respiratory system

respiratory system phenotype ( J:291983 )

N • normal lung histology

Genotype
MGI:6478907

cx2

• Allelic Composition: Dmd^mdx/Dmd^mdx; Snhg15^tm1Nju/Snhg15^tm1Nju
• Genetic Background: involves: C57BL/6J * C57BL/10ScSn

growth/size/body

growth/size/body region phenotype ( J:291983 )

N • normal body size and weight

muscle

muscle phenotype ( J:291983 )

N • normal tibialis anterior (TA) and gastrocnemius (GAS) muscle weight at age 8 weeks

increased variability of skeletal muscle fiber size ( J:291983 )

• more small diameter muscle fibers in tibialis anterior (TA) muscle weight at age 8 weeks

skeletal muscle fibrosis ( J:291983 )

• increased diaphragm muscle fibrosis at age 6-8 months

impaired skeletal muscle regeneration ( J:291983 )

• increased size of unrepaired areas in tibialis anterior (TA) muscle weight at age 8 weeks

• increased number of embryonic myosin heavy chain expressing myofibers in tibialis anterior (TA) muscle weight at age 8 weeks

• increased CD68+ macrophage infiltration in tibialis anterior (TA) muscle weight at age 8 weeks