Phenotypes associated with this allele

• Allele Symbol: Mink1^tm1.2Lgrl
• Allele Name: targeted mutation 1.2, Linrong Lu
• Allele ID: MGI:6474250
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mink1^tm1.2Lgrl/Mink1^tm1.2Lgrl	involves: 129S6/SvEvTac * C57BL/6	MGI:6474252

Genotype
MGI:6474252

hm1

• Allelic Composition: Mink1^tm1.2Lgrl/Mink1^tm1.2Lgrl
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

hematopoietic system phenotype ( J:231551 )

N • mice do not display increased bleeding tendency or thrombitic events over their lifespan

N • platelet counts, white cell counts, hematocrits and hemoglobin concentrations are similar to controls

N • platelets have normal discoid morphology with normal numbers of alpha- and dense-granules

abnormal platelet physiology ( J:231551 )

• display decreased spreading on immobilized fibrinogen

decreased platelet aggregation ( J:231551 )

• decrease in aggregation rates induced by thrombin (0.025U/ml), collagen (0.8 ug/ml), and TXA2 analog U46619 (0.3 uM)

• higher concentrations of thrombin, collagen, and TXA2 analog U46619 overcome the aggregation defect

• however, ADP-induced aggregation is similar to controls and supplementing with low concentrations of ADP rescues the aggregation defect

abnormal platelet dense granule physiology ( J:231551 )

• impaired dense granule secretion when stimulated by thrombin (0.025U/ml), collagen (0.8 ug/ml), and TXA2 analog U46619 (0.3 uM)

• higher concentrations of thrombin, collagen, and TXA2 analog U46619 do not overcome the secretion defect

homeostasis/metabolism

abnormal platelet physiology ( J:231551 )

• display decreased spreading on immobilized fibrinogen

decreased platelet aggregation ( J:231551 )

• decrease in aggregation rates induced by thrombin (0.025U/ml), collagen (0.8 ug/ml), and TXA2 analog U46619 (0.3 uM)

• higher concentrations of thrombin, collagen, and TXA2 analog U46619 overcome the aggregation defect

• however, ADP-induced aggregation is similar to controls and supplementing with low concentrations of ADP rescues the aggregation defect

abnormal platelet dense granule physiology ( J:231551 )

• impaired dense granule secretion when stimulated by thrombin (0.025U/ml), collagen (0.8 ug/ml), and TXA2 analog U46619 (0.3 uM)

• higher concentrations of thrombin, collagen, and TXA2 analog U46619 do not overcome the secretion defect

decreased susceptibility to induced thrombosis ( J:231551 )

• prolonged time to form a stable thrombosis in an FeCl3-induced mesenteric arteriole thrombosis model

• in an in vitro microfluidic whole-blood perfusion assay thrombi formed are significantly smaller compared to controls

increased bleeding time ( J:231551 )

• prolonged bleeding time in a tail-bleeding assay, with 48% of mice having a bleed time of over 15 minutes compared to 19% of wild-type controls