Analysis Tools
mortality/aging
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• pups start to die around 2 weeks of age, with about 60% lethality by P21-P25; pups that die develop a condition characterized by gradual weight loss, signs of dehydration and progressive lethargy
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• fewer (13% vs 25% expected) than the expected number of mice are born, indicating partial embryonic lethality
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growth/size/body
weight loss
(
J:287423
)
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• about 60% of pups show gradual weight loss between P15 and P21
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• pups show severe growth retardation
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digestive/alimentary system
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• tuft cells are almost entirely absent in the intestine
• however, goblet and enteroendocrine cell lineages are not altered
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• about 60% of mice exhibit a translucent and air-filled gut tube, particularly in the ileum, caecum, and colon
• tuft cells are almost entirely absent in the intestine
• however, goblet and enteroendocrine cell lineages are not altered
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• mice show altered crypt-villus architecture, particularly in the small intestine, with reduced number of crypts and smaller size
• average crypt depth is reduced
• loss of Lgr5+ intestinal stem cells
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• Paneth cell expansion is inhibited in the intestine
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• mice show delayed intestinal epithelial turnover
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• isolated crypts grown in culture show smaller structures and a slower growth rate but are able to generate organoids
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endocrine/exocrine glands
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• mice show altered crypt-villus architecture, particularly in the small intestine, with reduced number of crypts and smaller size
• average crypt depth is reduced
• loss of Lgr5+ intestinal stem cells
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• Paneth cell expansion is inhibited in the intestine
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homeostasis/metabolism
dehydration
(
J:287423
)
|
• about 60% of pups show signs of dehydration between P15 and P21
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cellular
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• tuft cells are almost entirely absent in the intestine
• however, goblet and enteroendocrine cell lineages are not altered
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• isolated crypts grown in culture show smaller structures and a slower growth rate but are able to generate organoids
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behavior/neurological
| N |
• pups show normal suckling behavior
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reproductive system
| N |
• both males and females are fertile
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