Phenotypes associated with this allele

• Allele Symbol: Tg(CAG-DPP4)52Ctkt
• Allele Name: transgene insertion 52, Chien-Te K Tseng
• Allele ID: MGI:6402051
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
tg1	Tg(CAG-DPP4)52Ctkt/0	involves: C3H/HeJ * C57BL/6J	MGI:6402055

Genotype
MGI:6402055

tg1

• Allelic Composition: Tg(CAG-DPP4)52Ctkt/0
• Genetic Background: involves: C3H/HeJ * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal mononuclear cell morphology ( J:227925 )

• mice infected with 10² TCID50 of MERS-CoV show mild and multifocal perivascular, peribronchial, and interstitial infiltrations with mononuclear cells at 2 and 4 dpi that increases further at 8 dpi

• focal mononuclear infiltrations are seen in the liver of mice infected with 10² TCID50 of MERS-CoV at 6 to 10 dpi

increased microglial cell activation ( J:227925 )

• mice infected with 10² TCID50 of MERS-CoV show microglia activation in brain stem tissues from 6 to 10 dpi

increased inflammatory response ( J:227925 )

• mild meningitis is seen in cerebral tissues of mice infected with 10² TCID50 of MERS-CoV from 8 to 10 dpi

brain inflammation ( J:227925 )

• mice infected with 10 LD50 of MERS-CoV show persistent inflammatory infiltrates in the brain stem at 2 and 6 days post infection (dpi), respectively

lung inflammation ( J:227925 )

• mice infected with a high dose of MERS-CoV develop progressive pneumonia, with extensive infiltration of inflammatory cells

• mice infected with 10² TCID50 of MERS-CoV show persistent inflammatory infiltrates in the lungs at 2 and 6 days post infection (dpi), respectively

• mice infected with 10² TCID50 of MERS-CoV show mild and multifocal perivascular, peribronchial, and interstitial infiltrations with mononuclear cells at 2 and 4 dpi that increases further at 8 dpi

interstitial pneumonia ( J:258199 )

• lungs of MERS-CoV infected mice exhibit gross lesions, bronchointerstitial pneumonitis and multifocal perivascular infiltrates that progress to more intense cellular infiltrates, including pulmonary macrophages and lymphocytes, within alveolar spaces

increased susceptibility to Coronaviridae infection ( J:227925 , J:258199 )

• the 50% infectious dose of MERS-CoV EMC/2012 strain is less than 1 TCID50 (50% tissue culture infectious doses per milliliter) (J:227925)

• mice surviving MERS-CoV infection develop serum neutralizing antibody and MERS-CoV S1 protein-specific IgG antibodies by 21 dpi and are fully immune to challenge with 10³ TCID50 (J:227925)

• most mice immunized with a MERS-CoV receptor binding domain-based subunit vaccine S377-588l-Fc plus MF59 adjuvant before being challenged with MERS-CoV recover from mild morbidity and survive (J:227925)

• intranasal administration of a fusion inhibitor peptide HR2M6 before challenge with MERS-CoV results in reduced lung viral titers and protection from death (J:227925)

• administration of HR2M6 after exposure to MERS-CoV does not provide therapeutic benefit (J:227925)

• mice are susceptible to infection with the EMC-2012 strain of the Middle East respiratory-coronavirus (MERS-CoV) and develop an acute wasting syndrome, with progressive weight loss starting at 2 days post infection (dpi), ruffled fur, lethargy, inactivity, rapid and shallow breathing, and mortality (J:258199)

• mice infected with MERS-CoV develop disseminated infection, with lung and brain being the prime sites of intense viral infection, and viral RNA detected in the heart, spleen, and intestine, but infectious virus could not be isolated from the heart, spleen, intestine, liver, and kidneys (J:258199)

• lung alveolar pneumocytes, both type I and type II, and brain microglia, astrocytes, and neuronal cells express abundant viral antigen at 2 and 4 dpi (J:258199)

increased susceptibility to Coronaviridae infection induced morbidity/mortality ( J:227925 , J:258199 )

• all mice infected with 10² to 10⁶ TCID50 of MERS-CoV-EMC/2012 strain succumb to infection (J:227925)

• 5 of 8 mice infected with a dose of 10¹ TCID50 of MERS-CoV die, with death occurring between 8-13 days post infection (dpi) (J:227925)

• the 50% lethal dose of MERS-CoV is 10 TCID50 (J:227925)

• mice infected with the EMC-2012 strain of the Middle East respiratory-coronavirus (MERS-CoV) show 30% mortality at 5 days post infection (dpi), 40% at 5 dpi, and 100% mortality at 6 dpi (J:258199)

growth/size/body

weight loss ( J:227925 , J:258199 )

• mice infected with 10³ TCID50 or higher of MERS-CoV show extreme weight loss of more than 20% while a dose of 10² results in less than 8% weight loss (J:227925)

• mice infected with a dose of 10¹ TCID50 of MERS-CoV show a 4% loss in weight (J:227925)

• MERS-CoV-infected mice exhibit progressive weight loss starting at 2 days post infection (dpi) (J:258199)

mortality/aging

increased susceptibility to Coronaviridae infection induced morbidity/mortality ( J:227925 , J:258199 )

• all mice infected with 10² to 10⁶ TCID50 of MERS-CoV-EMC/2012 strain succumb to infection (J:227925)

• 5 of 8 mice infected with a dose of 10¹ TCID50 of MERS-CoV die, with death occurring between 8-13 days post infection (dpi) (J:227925)

• the 50% lethal dose of MERS-CoV is 10 TCID50 (J:227925)

• mice infected with the EMC-2012 strain of the Middle East respiratory-coronavirus (MERS-CoV) show 30% mortality at 5 days post infection (dpi), 40% at 5 dpi, and 100% mortality at 6 dpi (J:258199)

hematopoietic system

abnormal mononuclear cell morphology ( J:227925 )

• mice infected with 10² TCID50 of MERS-CoV show mild and multifocal perivascular, peribronchial, and interstitial infiltrations with mononuclear cells at 2 and 4 dpi that increases further at 8 dpi

• focal mononuclear infiltrations are seen in the liver of mice infected with 10² TCID50 of MERS-CoV at 6 to 10 dpi

increased microglial cell activation ( J:227925 )

• mice infected with 10² TCID50 of MERS-CoV show microglia activation in brain stem tissues from 6 to 10 dpi

respiratory system

lung inflammation ( J:227925 )

• mice infected with a high dose of MERS-CoV develop progressive pneumonia, with extensive infiltration of inflammatory cells

• mice infected with 10² TCID50 of MERS-CoV show persistent inflammatory infiltrates in the lungs at 2 and 6 days post infection (dpi), respectively

• mice infected with 10² TCID50 of MERS-CoV show mild and multifocal perivascular, peribronchial, and interstitial infiltrations with mononuclear cells at 2 and 4 dpi that increases further at 8 dpi

interstitial pneumonia ( J:258199 )

• lungs of MERS-CoV infected mice exhibit gross lesions, bronchointerstitial pneumonitis and multifocal perivascular infiltrates that progress to more intense cellular infiltrates, including pulmonary macrophages and lymphocytes, within alveolar spaces

respiratory distress ( J:258199 )

• MERS-CoV-infected mice show rapid and shallow breathing

nervous system

increased microglial cell activation ( J:227925 )

• mice infected with 10² TCID50 of MERS-CoV show microglia activation in brain stem tissues from 6 to 10 dpi

brain inflammation ( J:227925 )

• mice infected with 10 LD50 of MERS-CoV show persistent inflammatory infiltrates in the brain stem at 2 and 6 days post infection (dpi), respectively

abnormal brainstem morphology ( J:227925 )

• mice infected with 10² TCID50 of MERS-CoV show perivascular cuffing, microglia activation, and apoptotic bodies in brain stem tissues from 6 to 10 dpi

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Middle East respiratory syndrome		DOID:0080642		J:227925 , J:258199