Phenotypes associated with this allele

• Allele Symbol: Prss55^tm1Zgwg
• Allele Name: targeted mutation 1, Zhugang Wang
• Allele ID: MGI:6357155
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Prss55^tm1Zgwg/Prss55^tm1Zgwg	involves: 129 * C57BL/6	MGI:6358239

Genotype
MGI:6358239

hm1

• Allelic Composition: Prss55^tm1Zgwg/Prss55^tm1Zgwg
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:278420 , J:278420 )

♀N • female fertility is normal (J:278420)

♂N • despite male infertility, no significant alterations in mating activity, sperm production, sperm morphology, sperm motility, acrosome reaction or activity of acrosomal enzymes are observed (J:278420)

impaired sperm migration in female genital tract ( J:278420 )

♂ • in vivo sperm migration in the female genital tract (from uterus into oviduct) is impaired

♂ • less than 10 sperm are present in the oviduct 3.5 h after coitus versus >300 sperm recovered in the oviduct of females mated with wild-type males

♂ • mature ADAM3 is nearly absent from cauda epididyma sperm, while its precursor form expressed in the testis is almost unaffected

male infertility ( J:278420 )

♂ • males are infertile; crossing of male homozygotes with wild-type females does not produce any offspring

♂ • however, the frequency of copulatory plug is normal

decreased fertilization frequency ( J:278420 )

♂ • in vivo fertilization rate of males is dramatically reduced to 4.1% versus 82.9% in wild-type controls

♂ • however, in vitro fertilization rate of sperm is comparable to that of wild-type sperm

impaired sperm-egg recognition ( J:278420 )

• severe defects in sperm-egg recognition in vitro

impaired binding of sperm to zona pellucida ( J:278420 )

♂ • severe defects in sperm binding to either zona-intact or zona-free oocytes in vitro

cellular

impaired sperm migration in female genital tract ( J:278420 )

♂ • in vivo sperm migration in the female genital tract (from uterus into oviduct) is impaired

♂ • less than 10 sperm are present in the oviduct 3.5 h after coitus versus >300 sperm recovered in the oviduct of females mated with wild-type males

♂ • mature ADAM3 is nearly absent from cauda epididyma sperm, while its precursor form expressed in the testis is almost unaffected