Phenotypes associated with this allele

• Allele Symbol: Slc9a8^{tm1c(KOMP)Wtsi}
• Allele Name: targeted mutation 1c, Wellcome Trust Sanger Institute
• Allele ID: MGI:5925353
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Slc9a8^tm1c(KOMP)Wtsi/Slc9a8^tm1c(KOMP)Wtsi Tg(Stra8-icre)1Reb/0	involves: C57BL/6J * C57BL/6N * FVB/NJ	MGI:6887371
cn2	Slc9a8^tm1c(KOMP)Wtsi/Slc9a8^tm1c(KOMP)Wtsi Tg(Amh-cre)8815Reb/0	involves: C57BL/6N	MGI:6887370

Genotype
MGI:6887371

cn1

• Allelic Composition: Slc9a8^{tm1c(KOMP)Wtsi}/Slc9a8^{tm1c(KOMP)Wtsi}; Tg(Stra8-icre)1Reb/0
• Genetic Background: involves: C57BL/6J * C57BL/6N * FVB/NJ
• Cell Lines: EPD0033_5_A07

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:245977 )

♂N • males show no significant changes in the morphology and diameter of seminiferous tubules relative to control males

♂N • ~5% of spermatozoa are NHE8-positive and show intact acrosomes and elongated nuclei in some tubules; this is attributed to the known ~95% deletion efficiency of Stra8-iCre mice

abnormal sperm mitochondrial sheath morphology ( J:245977 )

♂ • spermatozoa show abnormal mitochondrial distribution, similar to Slc9a8^{tm1d(KOMP)Wtsi} homozygotes

absent sperm mitochondrial sheath ( J:245977 )

♂ • MitoTracker staining revealed loss of the mitochondrial sheath, similar to Slc9a8^{tm1d(KOMP)Wtsi} homozygotes

abnormal sperm head morphology ( J:245977 )

♂ • in mature spermatozoa, DNA is condensed but sperm heads are not elongated and lack acrosomal caps

abnormal acrosome assembly ( J:245977 )

♂ • acrosome biogenesis is impaired, similar to Slc9a8^{tm1d(KOMP)Wtsi} homozygotes

abnormal proacrosomal vesicle fusion ( J:245977 )

♂ • although electron-dense vesicles accumulate between the Golgi and the nuclear envelope, no acrosomal granules or acrosomal caps are found, similar to Slc9a8^{tm1d(KOMP)Wtsi} homozygotes

globozoospermia ( J:245977 )

♂ • round-headed spermatozoa are observed, similar to Slc9a8^{tm1d(KOMP)Wtsi} homozygotes

absent acrosome ( J:245977 )

♂ • round-headed sperm lack acrosomes, similar to Slc9a8^{tm1d(KOMP)Wtsi} homozygotes

male infertility ( J:245977 )

♂ • males are infertile, similar to Slc9a8^{tm1d(KOMP)Wtsi} homozygotes

cellular

abnormal sperm mitochondrial sheath morphology ( J:245977 )

♂ • spermatozoa show abnormal mitochondrial distribution, similar to Slc9a8^{tm1d(KOMP)Wtsi} homozygotes

absent sperm mitochondrial sheath ( J:245977 )

♂ • MitoTracker staining revealed loss of the mitochondrial sheath, similar to Slc9a8^{tm1d(KOMP)Wtsi} homozygotes

abnormal sperm head morphology ( J:245977 )

♂ • in mature spermatozoa, DNA is condensed but sperm heads are not elongated and lack acrosomal caps

abnormal acrosome assembly ( J:245977 )

♂ • acrosome biogenesis is impaired, similar to Slc9a8^{tm1d(KOMP)Wtsi} homozygotes

abnormal proacrosomal vesicle fusion ( J:245977 )

♂ • although electron-dense vesicles accumulate between the Golgi and the nuclear envelope, no acrosomal granules or acrosomal caps are found, similar to Slc9a8^{tm1d(KOMP)Wtsi} homozygotes

globozoospermia ( J:245977 )

♂ • round-headed spermatozoa are observed, similar to Slc9a8^{tm1d(KOMP)Wtsi} homozygotes

absent acrosome ( J:245977 )

♂ • round-headed sperm lack acrosomes, similar to Slc9a8^{tm1d(KOMP)Wtsi} homozygotes

Genotype
MGI:6887370

cn2

• Allelic Composition: Slc9a8^{tm1c(KOMP)Wtsi}/Slc9a8^{tm1c(KOMP)Wtsi}; Tg(Amh-cre)8815Reb/0
• Genetic Background: involves: C57BL/6N
• Cell Lines: EPD0033_5_A07

reproductive system

reproductive system phenotype ( J:245977 )

♂N • males are fertile and show no significant changes in the morphology and diameter of seminiferous tubules or sperm morphology relative to control males; spermatozoa exhibit normal acrosomal caps and mitochondrial sheaths