Phenotypes associated with this allele

• Allele Symbol: Polr1a^{tm1b(EUCOMM)Hmgu}
• Allele Name: targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• Allele ID: MGI:5810733
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Polr1a^tm1b(EUCOMM)Hmgu/Polr1a^tm1b(EUCOMM)Hmgu	B6N(Cg)-Polr1a^tm1b(EUCOMM)Hmgu/Bay	MGI:6377022
hm2	Polr1a^tm1b(EUCOMM)Hmgu/Polr1a^tm1b(EUCOMM)Hmgu	C57BL/6N-Polr1a^tm1b(EUCOMM)Hmgu/Bay	MGI:6263201

Genotype
MGI:6377022

hm1

• Allelic Composition: Polr1a^{tm1b(EUCOMM)Hmgu}/Polr1a^{tm1b(EUCOMM)Hmgu}
• Genetic Background: B6N(Cg)-Polr1a^{tm1b(EUCOMM)Hmgu}/Bay
• Cell Lines: HEPD0779_7_B03

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality before implantation, complete penetrance ( J:279207 )

• only 3% of homozygous embryos are recovered at E3.5 as dying 2-cell stage embryos; no embryos or excess resorption sites are observed at E7.5

embryo

failure of blastocyst formation ( J:279207 )

• 2-cell stage embryos fail to reach a morphological blastocyst stage after 3 days in culture

failure of zygotic cell division ( J:279207 )

• only dying 2-cell stage embryos are observed at E3.5, suggesting failure of zygotic genome activation

cellular

increased cell death ( J:279207 )

• 2-cell stage embryos recovered at E3.5 appear to be dying

Genotype
MGI:6263201

hm2

• Allelic Composition: Polr1a^{tm1b(EUCOMM)Hmgu}/Polr1a^{tm1b(EUCOMM)Hmgu}
• Genetic Background: C57BL/6N-Polr1a^{tm1b(EUCOMM)Hmgu}/Bay
• Cell Lines: HEPD0779_7_B03

Data Sources

IMPC Data for Polr1a

mortality/aging

preweaning lethality, complete penetrance ( J:211773 )

IMPC - BCM