Phenotypes associated with this allele
|
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gpx4tm4Marc mutation
(0 available);
any
Gpx4 mutation
(80 available)
|
|
|
Gpx4tm4Marc/Gpx4tm4Marc embryos show resorptions and severe abnormalities of embryonic and extraembryonic structures
mortality/aging
|
|
• homozygous embryos die at E7.5-E8.5
• an increased number of resorptions and empty decidua, but no viable embryos, are noted at E9.5
|
embryo
|
|
• a clear distinction of the three germinal layers is not possible
|
|
|
• severe abnormalities in embryonic structures
|
|
|
• severe abnormalities in extraembryonic structures
|
reproductive system
Allelic
Composition |
Gpx4tm4Marc/Gpx4+
|
|
Genetic
Background |
involves: 129S6/SvEvTac * C57BL/6J |
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gpx4tm4Marc mutation
(0 available);
any
Gpx4 mutation
(80 available)
|
|
|
Severe morphological alterations in the midpiece of developing and mature sperm in Gpx4tm4Marc/Gpx4+ mice
reproductive system
 |
• immunocytochemical staining of whole-mount spermatozoa revealed elevated Gpx4 expression particularly in the midpiece of isolated spermatozoa
• many severe morphological defecs are observed in the midpiece and midpiece-principal piece junction of developing and mature sperm
• aberrations include bends mainly at the midpiece-principal piece junction, irregularly aligned mitochondria, and protrusions of outer dense fibers
|
|
|
• late stage spermatids and epididymal spermatozoa exhibit swollen mitochondria surrounding the axoneme
|
|
|
• bends mainly at the midpiece-principal piece junction
|
|
|
• immunocytochemical staining of whole-mount spermatozoa revealed elevated Gpx4 expression in the head of isolated spermatozoa
|
|
|
• heterozygous males yield litter sizes ranging from 2 to 10 pups per litter relative to 1 to 11 pups per litter in wild-type controls
|
|
|
• male heterozygotes are subfertile in conventional breeding and in vitro fertilization assays
• test breeding of heterozygous males with wild-type C57BL/6J females yielded 11 litters with a total number of 70 offspring whereas breeding of wild-type males yielded 17 litters with a total number of 119 offspring
|
|
|
• in vitro fertilization assays using heterozygous mutant sperm and wild-type oocytes revealed a significant reduction in the number of zygotes (pro-nuclei stage embryos) and developed blastocysts (16 zygotes; 2 blastocysts) relative to wild-type controls (105 zygotes; 20 blastocysts)
|
|
|
• in vitro fertilization assays using heterozygous mutant sperm and wild-type oocytes revealed a significant reduction in the number of zygotes (pro-nuclei stage embryos) and developed blastocysts (16 zygotes; 2 blastocysts) relative to wild-type controls (105 zygotes; 20 blastocysts)
|
|
|
• sperm isolated from the cauda epididymis exhibit higher levels of free reduced thiols than wild-type sperm
|
|
|
• spermatozoa from heterozygous males exhibit a decrease in overall sperm progressivity
• however, total sperm concentration and motility are normal
|
cellular
|
|
• immunocytochemical staining of whole-mount spermatozoa revealed elevated Gpx4 expression particularly in the midpiece of isolated spermatozoa
• many severe morphological defecs are observed in the midpiece and midpiece-principal piece junction of developing and mature sperm
• aberrations include bends mainly at the midpiece-principal piece junction, irregularly aligned mitochondria, and protrusions of outer dense fibers
|
|
|
• late stage spermatids and epididymal spermatozoa exhibit swollen mitochondria surrounding the axoneme
|
|
|
• bends mainly at the midpiece-principal piece junction
|
|
|
• immunocytochemical staining of whole-mount spermatozoa revealed elevated Gpx4 expression in the head of isolated spermatozoa
|
|
|
• spermatozoa from heterozygous males exhibit a decrease in overall sperm progressivity
• however, total sperm concentration and motility are normal
|
Analysis Tools