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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Ppp2catm1.2Jmli
targeted mutation 1.2, Jianmin Li
MGI:5755280
Summary 1 genotype
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Ppp2catm1.1Jmli/Ppp2catm1.2Jmli
Tg(Ddx4-cre)1Dcas/0
involves: C57BL/6J * FVB MGI:5795985


Genotype
MGI:5795985
cn1
Allelic
Composition
Ppp2catm1.1Jmli/Ppp2catm1.2Jmli
Tg(Ddx4-cre)1Dcas/0
Genetic
Background
involves: C57BL/6J * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ppp2catm1.1Jmli mutation (0 available); any Ppp2ca mutation (25 available)
Ppp2catm1.2Jmli mutation (0 available); any Ppp2ca mutation (25 available)
Tg(Ddx4-cre)1Dcas mutation (2 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
reproductive system
• at 4 weeks of age, seminiferous tubules exhibit vacuolation and disrupted arrangement of spermatogonia and primary spermatocytes
• by 8 weeks of age, fewer vacuole-like structures are found, germ cells appear disorganized, and elongated spermatozoa are absent
• at 4 weeks of age, seminiferous tubules exhibit vacuolation
• however, fewer vacuole-like structures are found by 8 weeks of age
• at 4 weeks of age, testes are significantly smaller than wild-type testes
• at 4 weeks of age, testes are ~20% the weight of control testes
• by 8 weeks of age, testes are ~50% the weight of control testes
• by 8 weeks of age, testes are almost completely devoid of mature spermatozoa, as shown by H&E staining of the seminiferous tubules and epididymides
• spermatogenesis is disrupted at all levels, as germ cells are either arranged in a disorderly manner or completely absent
• no normal sperm are observed in a sperm smear collected from P56 epididymides
• at P38, flow cytometric analysis revealed that meiosis is blocked at the diploid stage
• surprisingly, the numbers of haploid and diploid cells are almost identical at P56, indicating that some cells can proceed to the next stage; however, any haploid cells still present at P56 appear abnormal
• by 8 weeks of age, cauda epididymides contain some round cells of different sizes and shapes but no normal sperm
• males fail to produce pups

endocrine/exocrine glands
• at 4 weeks of age, seminiferous tubules exhibit vacuolation and disrupted arrangement of spermatogonia and primary spermatocytes
• by 8 weeks of age, fewer vacuole-like structures are found, germ cells appear disorganized, and elongated spermatozoa are absent
• at 4 weeks of age, seminiferous tubules exhibit vacuolation
• however, fewer vacuole-like structures are found by 8 weeks of age
• at 4 weeks of age, testes are significantly smaller than wild-type testes
• at 4 weeks of age, testes are ~20% the weight of control testes
• by 8 weeks of age, testes are ~50% the weight of control testes

cellular
• at P38, flow cytometric analysis revealed that meiosis is blocked at the diploid stage
• surprisingly, the numbers of haploid and diploid cells are almost identical at P56, indicating that some cells can proceed to the next stage; however, any haploid cells still present at P56 appear abnormal





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last database update
04/30/2024
MGI 6.23
The Jackson Laboratory