Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Best1tm1Web mutation
(1 available);
any
Best1 mutation
(27 available)
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reproductive system
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• Background Sensitivity: on a C57BL/6 background the fraction of sperm with tail abnormalities is lower than on a CD-1 background
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• a high number of caudal spermatozoa exhibit a thickened acrosome relative to wild-type controls
• Background Sensitivity: on a C57BL/6 background sperm are less vesiculated and less acrosome-reacted than on a CD-1 background
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• litters sired by male homozygotes mated to wild-type females are reduced (4 +/- 2.2 vs 8.5 +/- 2.2 pups/litter for wild-type males)
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• male homozygotes display severe subfertility despite normal mating behavior
• Background Sensitivity: male subfertility is even more pronounced on a CD-1 genetic background
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cellular
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• Background Sensitivity: on a C57BL/6 background the fraction of sperm with tail abnormalities is lower than on a CD-1 background
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• a high number of caudal spermatozoa exhibit a thickened acrosome relative to wild-type controls
• Background Sensitivity: on a C57BL/6 background sperm are less vesiculated and less acrosome-reacted than on a CD-1 background
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vision/eye
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N |
• extensive analysis of retinal and RPE integrity revealed no signs of histological or functional retinal pathology
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Best1tm1Web mutation
(1 available);
any
Best1 mutation
(27 available)
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Sperm abnormalities in Best1tm1Web/Best1tm1Web males
reproductive system
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• a large fraction of sperm display severe coiling of the flagellum, suggesting impaired sperm volume regulation
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• a high percentage of sperm exhibit heads without tails, indicating enhanced decapitation
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• a large fraction of sperm display angulation of the flagellum
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• 30% decline in cauda sperm viability
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• increase in the number of acrosome-reacted, -vesiculated, or -thickened acrosomal structures
• the fraction of acrosome intact spermatozoa declines drastically as spermatozoa progress from the caput to the corpus and cauda region of the epididymal duct
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• increase in the number of acrosome-reacted (perforated or absent acrosome cap) sperm
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• matings between male homozygotes and wild-type females result in only 7 pups born out of 23 crosses (0.3 pups per mating), whereas wild-type breedings yield an average of 12.1 pups per mating
• Background Sensitivity: male subfertility is less severe on a C57BL/6 background than on a CD-1 background (following at least 10 backcrosses onto the CD-1 strain)
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• caudal sperm show impaired tolerance to changes in osmotic pressure
• however, basal cytosolic Ca2+ levels [Ca2+]i, ATP-induced, and swelling-activated increases in [Ca2+]i are similar to those in wild-type controls, indicating normal Ca2+ homeostasis
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• severe decrease in all sperm motility parameters analyzed (including curvilinear velocity, average path velocity, straight line velocity, and amplitude of lateral head displacement) relative to wild-type sperm
• partial rescue of total motility in caudal spermatozoa after exposure to solutions with increasing osmolality (THY290, 16 +/- 4%; THY350, 20 +/- 7%; THY420, 31 +/- 6%), although percentage of total motility is significantly lower at all osmotic conditions relative to wild-type controls (22 +/- 8% vs. 65 +/- 7%)
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cellular
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• a large fraction of sperm display severe coiling of the flagellum, suggesting impaired sperm volume regulation
|
|
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• a high percentage of sperm exhibit heads without tails, indicating enhanced decapitation
|
|
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• a large fraction of sperm display angulation of the flagellum
|
|
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• 30% decline in cauda sperm viability
|
|
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• increase in the number of acrosome-reacted, -vesiculated, or -thickened acrosomal structures
• the fraction of acrosome intact spermatozoa declines drastically as spermatozoa progress from the caput to the corpus and cauda region of the epididymal duct
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• increase in the number of acrosome-reacted (perforated or absent acrosome cap) sperm
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• severe decrease in all sperm motility parameters analyzed (including curvilinear velocity, average path velocity, straight line velocity, and amplitude of lateral head displacement) relative to wild-type sperm
• partial rescue of total motility in caudal spermatozoa after exposure to solutions with increasing osmolality (THY290, 16 +/- 4%; THY350, 20 +/- 7%; THY420, 31 +/- 6%), although percentage of total motility is significantly lower at all osmotic conditions relative to wild-type controls (22 +/- 8% vs. 65 +/- 7%)
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vision/eye
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N |
• extensive analysis of retinal and RPE integrity revealed no signs of histological or functional retinal pathology
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Analysis Tools