Phenotypes associated with this allele

• Allele Symbol: Adgrf5^tm1.2Jpbs
• Allele Name: targeted mutation 1.2, James P Bridges
• Allele ID: MGI:5697003
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Adgrf5^tm1.2Jpbs/Adgrf5^tm1.2Jpbs	BALB/c-Adgrf5^tm1.2Jpbs	MGI:5697400

Genotype
MGI:5697400

hm1

• Allelic Composition: Adgrf5^tm1.2Jpbs/Adgrf5^tm1.2Jpbs
• Genetic Background: BALB/c-Adgrf5^tm1.2Jpbs

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

growth/size/body region phenotype ( J:221769 )

N • body weight normal from E16.5 through 12 weeks of age

respiratory system

abnormal lung morphology ( J:221769 )

• focal clusters of subpleural lymphoid cells at 20 weeks of age, mostly B cells

abnormal alveolar macrophage morphology ( J:221769 )

• lipid containing macrophage at 20 weeks

abnormal pulmonary alveolus morphology ( J:221769 )

• distal air spaces are enlarged at 4 and 20 weeks of age

abnormal respiratory system physiology ( J:221769 )

abnormal respiration ( J:221769 )

tachypnea ( J:221769 )

respiratory distress ( J:221769 )

• signs of severe respiratory distress develop between 20 and 32 weeks of age

• chest retractions

abnormal surfactant physiology ( J:221769 )

• synthesis of SatPC increased 2 fold at 4 weeks which is typical for all phospholipid surfactants

abnormal surfactant composition ( J:221769 )

• broncho-alveolar lavage fluid from 20 week mice is turbid and viscous

• surfactant protein B and surfactant protein C are increased in alveolar fluid

• surfactant protein A decreased and surfactant protein D increased in lung tissue

• total protein content of broncho-alveolar fluid is increased 2.6%

increased surfactant secretion ( J:221769 )

• saturated phosphatidylcholine (SatPC) levels are similar to controls at E18.5

• significantly elevated SatPC levels at 4 weeks, by 13.9X in alveolar fluid and 3.7X in tissue

• elevated SatPC by 33.3X in alveolar fluid at 20weeks and 29.8X in tissue

• secreted levels from cells in culture increased 1.9X

immune system

abnormal alveolar macrophage morphology ( J:221769 )

• lipid containing macrophage at 20 weeks

hematopoietic system

abnormal alveolar macrophage morphology ( J:221769 )

• lipid containing macrophage at 20 weeks