Phenotypes associated with this allele

• Allele Symbol: Rack1^tm1Sbif
• Allele Name: targeted mutation 1, Stefano Biffo
• Allele ID: MGI:5660834
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rack1^tm1Sbif/Rack1^tm1Sbif	C57BL/6-Rack1^tm1Sbif	MGI:5779955
ht2	Rack1^tm1Sbif/Rack1^+	C57BL/6-Rack1^tm1Sbif	MGI:5779956

Genotype
MGI:5779955

hm1

• Allelic Composition: Rack1^tm1Sbif/Rack1^tm1Sbif
• Genetic Background: C57BL/6-Rack1^tm1Sbif

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and placentation, complete penetrance ( J:223207 )

• homozygotes die at gastrulation; mutant embryos are resorbed at E9.5

embryo

abnormal embryo development ( J:223207 )

• disorganized implanted blastocysts with vacuolar areas are observed at E6.5

absent egg cylinders ( J:223207 )

• absence of the early egg cylinder at E6.5

Genotype
MGI:5779956

ht2

• Allelic Composition: Rack1^tm1Sbif/Rack1⁺
• Genetic Background: C57BL/6-Rack1^tm1Sbif

pigmentation

belly spot ( J:223207 )

• white belly spot in adult mice

decreased foot pigmentation ( J:223207 )

• hypopigmented paws in adult mice

decreased tail pigmentation ( J:223207 )

non-pigmented tail tip ( J:223207 )

• hypopigmented tail tips in adult mice

growth/size/body

decreased body weight ( J:223207 )

♀ • transient decrease in body weight in females but not males at P11

decreased body length ( J:223207 )

♀ • transient decrease in head-anus length in females but not males at P11

postnatal growth retardation ( J:223207 )

♀ • transient growth delay in young females, not seen in males

♀ • however, no gross alterations in embryonic development

homeostasis/metabolism

abnormal translation ( J:223207 )

• reduced translation (protein synthesis) in response to stimulation with insulin or the PKC agonist PMA in heterozygous mutant MEFs relative to wild-type MEFs

• mildly increased 80S/polysome ratio in heterozygous mutant livers relative to wild-type livers

limbs/digits/tail

decreased tail pigmentation ( J:223207 )

non-pigmented tail tip ( J:223207 )

• hypopigmented tail tips in adult mice

integument

belly spot ( J:223207 )

• white belly spot in adult mice

decreased foot pigmentation ( J:223207 )

• hypopigmented paws in adult mice

decreased tail pigmentation ( J:223207 )

non-pigmented tail tip ( J:223207 )

• hypopigmented tail tips in adult mice

cellular

abnormal translation ( J:223207 )

• reduced translation (protein synthesis) in response to stimulation with insulin or the PKC agonist PMA in heterozygous mutant MEFs relative to wild-type MEFs

• mildly increased 80S/polysome ratio in heterozygous mutant livers relative to wild-type livers