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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tg(tetO-Kras*G12D)#Rdp
transgene insertion, Ronald DePinho
MGI:5648530
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/Gt(ROSA)26Sor+
Ptf1atm1.1(cre)Cvw/Ptf1a+
Tg(tetO-Kras*G12D)#Rdp/0
Trp53tm1Brn/Trp53+ 		involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N MGI:5648533
cn2
 		Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/Gt(ROSA)26Sor+
Ptf1atm1.1(cre)Cvw/Ptf1a+
Tg(tetO-Kras*G12D)#Rdp/0
Trp53tm1Brn/Trp53tm1Brn 		involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N MGI:5648534
cn3
 		Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/Gt(ROSA)26Sor+
Ptf1atm1.1(cre)Cvw/Ptf1a+
Tg(tetO-Kras*G12D)#Rdp/0 		involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N MGI:5648532


Genotype
MGI:5648533
cn1
Allelic
Composition		 Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/Gt(ROSA)26Sor+
Ptf1atm1.1(cre)Cvw/Ptf1a+
Tg(tetO-Kras*G12D)#Rdp/0
Trp53tm1Brn/Trp53+
Genetic
Background		 involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy mutation (5 available); any Gt(ROSA)26Sor mutation (944 available)
Ptf1atm1.1(cre)Cvw mutation (1 available); any Ptf1a mutation (30 available)
Tg(tetO-Kras*G12D)#Rdp mutation (0 available)
Trp53tm1Brn mutation (18 available); any Trp53 mutation (232 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
neoplasm
increased pancreatic ductal adenocarcinoma incidence ( J:186194 )
• all mice succumb to pancreatic ductal adenocarcinoma (PDAC) between 11 and 25 weeks of age following doxycycline treatment at 3 weeks of age
• tumors exhibit features of human PDAC, including glandular tumor structures, exuberant stroma, local invasion into surrounding structures such as the duodenum, and distant metastases to the liver and lung
• mice show rapid tumor regression staring 48 hours and peaking at 72 hours following doxycycline withdrawal, with a reduction in tumor mass of about 50% after 1 week of doxycycline withdrawal; tumor regression is accompanied by decreased tumor cell proliferation and increased apoptosis
• withdrawal of doxycycline results in decreased glucose uptake and lactate production by cells in vitro

endocrine/exocrine glands
increased pancreatic ductal adenocarcinoma incidence ( J:186194 )
• all mice succumb to pancreatic ductal adenocarcinoma (PDAC) between 11 and 25 weeks of age following doxycycline treatment at 3 weeks of age
• tumors exhibit features of human PDAC, including glandular tumor structures, exuberant stroma, local invasion into surrounding structures such as the duodenum, and distant metastases to the liver and lung
• mice show rapid tumor regression staring 48 hours and peaking at 72 hours following doxycycline withdrawal, with a reduction in tumor mass of about 50% after 1 week of doxycycline withdrawal; tumor regression is accompanied by decreased tumor cell proliferation and increased apoptosis
• withdrawal of doxycycline results in decreased glucose uptake and lactate production by cells in vitro

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
pancreatic carcinoma DOID:4905 OMIM:260350
 J:186194




Genotype
MGI:5648534
cn2
Allelic
Composition		 Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/Gt(ROSA)26Sor+
Ptf1atm1.1(cre)Cvw/Ptf1a+
Tg(tetO-Kras*G12D)#Rdp/0
Trp53tm1Brn/Trp53tm1Brn
Genetic
Background		 involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy mutation (5 available); any Gt(ROSA)26Sor mutation (944 available)
Ptf1atm1.1(cre)Cvw mutation (1 available); any Ptf1a mutation (30 available)
Tg(tetO-Kras*G12D)#Rdp mutation (0 available)
Trp53tm1Brn mutation (18 available); any Trp53 mutation (232 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
neoplasm
increased pancreatic ductal adenocarcinoma incidence ( J:186194 )
• all mice succumb to pancreatic ductal adenocarcinoma (PDAC) at a median age of 7.9 weeks following doxycycline treatment at 3 weeks of age

endocrine/exocrine glands
increased pancreatic ductal adenocarcinoma incidence ( J:186194 )
• all mice succumb to pancreatic ductal adenocarcinoma (PDAC) at a median age of 7.9 weeks following doxycycline treatment at 3 weeks of age

Mouse Models of Human Disease
 DO ID OMIM ID(s) Ref(s)
pancreatic carcinoma DOID:4905 OMIM:260350
 J:186194




Genotype
MGI:5648532
cn3
Allelic
Composition		 Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/Gt(ROSA)26Sor+
Ptf1atm1.1(cre)Cvw/Ptf1a+
Tg(tetO-Kras*G12D)#Rdp/0
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy mutation (5 available); any Gt(ROSA)26Sor mutation (944 available)
Ptf1atm1.1(cre)Cvw mutation (1 available); any Ptf1a mutation (30 available)
Tg(tetO-Kras*G12D)#Rdp mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
endocrine/exocrine glands
pancreatic acinar-to-ductal metaplasia ( J:186194 )
• mice develop acinar-to-ductal metaplasia within 2 weeks of doxycycline induction
increased pancreatic ductal adenocarcinoma incidence ( J:186194 )
• infrequently, mice exhibit occurrence of invasive pancreatic ductal adenocarcinoma after long latency (35-70 weeks) following doxycline treatment
increased pancreatic intraepithelial neoplasia incidence ( J:186194 )
• mice develop PanIN lesions within 2 weeks of doxycycline induction

neoplasm
increased pancreatic ductal adenocarcinoma incidence ( J:186194 )
• infrequently, mice exhibit occurrence of invasive pancreatic ductal adenocarcinoma after long latency (35-70 weeks) following doxycline treatment
increased pancreatic intraepithelial neoplasia incidence ( J:186194 )
• mice develop PanIN lesions within 2 weeks of doxycycline induction




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04/30/2024
MGI 6.23 		The Jackson Laboratory