Phenotypes associated with this allele

• Allele Symbol: Cyp2c23^tm1.1Jhc
• Allele Name: targeted mutation 1.1, Jorge H Capdevila
• Allele ID: MGI:5638557
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Cyp2c23^tm1.1Jhc/Cyp2c23^tm1.1Jhc Tg(Hoxb7-cre)13Amc/0	involves: C57BL/6	MGI:5695613

Genotype
MGI:5695613

cn1

• Allelic Composition: Cyp2c23^tm1.1Jhc/Cyp2c23^tm1.1Jhc; Tg(Hoxb7-cre)13Amc/0
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

increased body weight ( J:213555 )

• mutant mice fed a high K+ (5% KCl) diet for 3 days show a significantly increased body weight on HK day 2, unlike wild-type controls where increased K+ intake has no effect on body weight

cardiovascular system

increased systemic arterial systolic blood pressure ( J:213555 )

• mutant mice fed a high K+ (5% KCl) diet display significantly increased systolic BP and remain hypertensive for 3 days during increased K+ intake, unlike wild-type controls

• addition of amiloride (an epithelial Na+ channel inhibitor) in drinking water abolishes high K+ intake-induced hypertension and decreases systolic BP from 146 +/- 5 to 117 +/- 3 mmHg, unlike in wild-type controls where amiloride has no effect on BP

• mutant mice fed a high-salt (4% NaCl) diet for 3 days show no significant differences in mean systolic BP relative to wild-type controls

• no differences in mean systolic BP are observed under normal salt or normal K+ (1.0% KCl) conditions relative to wild-type controls

renal/urinary system

decreased urine sodium level ( J:213555 )

• mutant mice fed a high K+ (5% KCl) diet display significantly decreased Na+ excretion relative to mutant mice fed a normal K+ (1.0% KCl) diet, unlike wild-type controls

• however, mutant mice exhibit no differences in Na+ intake when fed a 5% KCl diet or a normal (1.0% KCl) diet

abnormal kidney physiology ( J:213555 )

• 10 uM of arachidonic acid (AA) fails to inhibit apical epithelial Na+ channel (ENaC) activity in the cortical collecting duct (CCD) of mutant mice fed a high K+ (5% KCl) diet, unlike in wild-type controls where channel activity (NPo) is decreased from 1.2 +/- 0.2 to 0.3 +/- 0.1

• however, addition of 100 nM 11,12-epoxyeicosatrienoic acid (11,12-EET) is still able to inhibit ENaC in the CCD of mutant mice fed a high K+ (5% KCl) diet, with NPo reduced from 2.5 +/- 0.4 to 0.2 +/- 0.1

homeostasis/metabolism

decreased urine sodium level ( J:213555 )

• mutant mice fed a high K+ (5% KCl) diet display significantly decreased Na+ excretion relative to mutant mice fed a normal K+ (1.0% KCl) diet, unlike wild-type controls

• however, mutant mice exhibit no differences in Na+ intake when fed a 5% KCl diet or a normal (1.0% KCl) diet