Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slc12a5tm2Hsav mutation
(0 available);
any
Slc12a5 mutation
(43 available)
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nervous system
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• primary cultures of cortical neurons show longer dendritic protrusions of pyramidal-shaped neurons than in wild-type cultures, with varied morphology from filopodia-like structures to protrusions with clear bulbous heads
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• the number of functional dendritic synapses is lower in neurons
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• lower frequency of miniature excitatory postsynaptic currents (mEPSCs) in neurons but no difference in amplitude
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Allelic
Composition |
Slc12a5tm1Hsav/Slc12a5tm2Hsav
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Genetic
Background |
involves: 129S1/Sv * 129S2/SvPasHsd * 129X1/SvJ * C57BL/6J * C57BL/6JOlaHsd |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slc12a5tm1Hsav mutation
(0 available);
any
Slc12a5 mutation
(43 available)
Slc12a5tm2Hsav mutation
(0 available);
any
Slc12a5 mutation
(43 available)
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growth/size/body
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• mice exhibit normal weight at birth, however, by 2 weeks of age, they weigh about 20% less than wild-type mice and this lower weight persists until at least 7 months of age
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behavior/neurological
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• mutants are hypersensitive to PTZ-induced seizures, showing increased latency to clonic seizures, longer seizure duration, and shorter latency to lethality
• however, mice do not develop spontaneous seizures
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• rearing activity of mutants did not habituate in the open field as in controls which show more rearings at the beginning than the end of the test session
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• in the water maze, mice show a longer cumulative distance to target than controls during the learning period
• in a subsequent transfer test, mutants swim further from the target than wild-type mice
• reversal learning shows decreased ability to locate the platform
• in the second transfer test after reversal learning, mutants are closer to the previous platform location and further away from the new place than the wild-type mice
• however, mutants are able to learn the simple visually cued escape from the water maze
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• in the elevated plus-maze, mice avoid entering the open arms almost completely, make less exploratory rearings and head dippings, and defecate more often than wild-type littermates
• although mice show normal exploration in the light-dark test, they show less rearings and defecate more frequently than wild-type mice
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• mice are hyposensitive to tactile stimulation, with a lower percentage of mutants responding to a given stimulus force with calibrated von Frey hairs
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• mice show less rearings during the elevated plus-maze, in the light-dark exploration test, and in the Y-maze test
• mice however, exhibit normal spontaneous locomotor activity and motor coordination in the open field, Y-maze, rotarod, and beam tests
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• mice are hyposensitive to thermal stimulation, showing decreased sensitivity to lower temperatures in the tail withdrawal and hot-plate tests
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nervous system
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N |
• mice do not show gross changes in brain histology
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• mutants are hypersensitive to PTZ-induced seizures, showing increased latency to clonic seizures, longer seizure duration, and shorter latency to lethality
• however, mice do not develop spontaneous seizures
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reproductive system
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slc12a5tm1Hsav mutation
(0 available);
any
Slc12a5 mutation
(43 available)
Slc12a5tm2Hsav mutation
(0 available);
any
Slc12a5 mutation
(43 available)
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nervous system
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• dendritic protrusions are longer in neurons, however, no difference is protrusion density is seen
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Analysis Tools