Phenotypes associated with this allele

• Allele Symbol: Tg(Th-cre)#Gsat
• Allele Name: transgene insertion, GENSAT Project at Rockefeller University
• Allele ID: MGI:5607431
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Trp53inp2^tm1c(KOMP)Mbp/Trp53inp2^tm1c(KOMP)Mbp Tg(Th-cre)#Gsat/0	involves: 129S4/SvJaeSor * C57BL/6N * FVB/N	MGI:6507407
cn2	Wnt5a^tm1.1Krvl/Wnt5a^tm1.1Krvl Tg(Th-cre)#Gsat/0	involves: 129S6/SvEvTac * C57BL/6 * FVB/N	MGI:5607432

Genotype
MGI:6507407

cn1

• Allelic Composition: Trp53inp2^{tm1c(KOMP)Mbp}/Trp53inp2^{tm1c(KOMP)Mbp}; Tg(Th-cre)#Gsat/0
• Genetic Background: involves: 129S4/SvJaeSor * C57BL/6N * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal axon extension ( J:276249 )

• defects in axon growth of NGF-responsive sympathetic neurons are observed in the heart at E16.5

abnormal superior cervical ganglion morphology ( J:276249 )

abnormal sympathetic neuron morphology ( J:276249 )

• analysis of superior cervical ganglia (SCGs) revealed normal numbers of sympathetic neurons at E16.5 but significant cell loss at birth (P0.5) and 3 weeks after birth (P21) relative to control littermates

abnormal sympathetic neuron innervation pattern ( J:276249 )

• although no neuronal loss is detected at E16.5, sympathetic innervation of the heart is already decreased with a significant reduction in both density of innervation and axonal branching

decreased sensory neuron number ( J:276249 )

• analysis of superior cervical ganglia (SCGs) revealed normal numbers of sympathetic neurons at E16.5 but significant cell loss at birth (P0.5) and 3 weeks after birth (P21) relative to control littermates

• neuronal loss is likely due to the axons failing to reach target tissues and gain access to adequate levels of nerve growth factor (NGF)

cellular

abnormal axon extension ( J:276249 )

• defects in axon growth of NGF-responsive sympathetic neurons are observed in the heart at E16.5

Genotype
MGI:5607432

cn2

• Allelic Composition: Wnt5a^tm1.1Krvl/Wnt5a^tm1.1Krvl; Tg(Th-cre)#Gsat/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * FVB/N

nervous system

abnormal superior cervical ganglion morphology ( J:196291 )

abnormal sympathetic neuron innervation pattern ( J:196291 )

• reduced innervation of peripheral tissues

decreased neuron number ( J:196291 )

• 30% decrease in numbers of superior cervical ganglia neurons by P7