Phenotypes associated with this allele

• Allele Symbol: Trim30a^tm1Yjk
• Allele Name: targeted mutation 1, Young-Joon Kim
• Allele ID: MGI:5606169
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Trim30a^tm1Yjk/Trim30a^tm1Yjk	B6.129P2-Trim30a^tm1Yjk	MGI:5606170
cx2	Tg(TcraTcrb)425Cbn/0 Trim30a^tm1Yjk/Trim30a^tm1Yjk	involves: 129P2/OlaHsd * BALB/c * C57BL/6	MGI:5606171

Genotype
MGI:5606170

hm1

• Allelic Composition: Trim30a^tm1Yjk/Trim30a^tm1Yjk
• Genetic Background: B6.129P2-Trim30a^tm1Yjk

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased T cell proliferation ( J:215171 )

• CD4+ T cells show hyper-proliferation when stimulated with anti-CD3 antibody (TCR activation), but not when stimulated with PMA and ionomycin

abnormal T cell subpopulation ratio ( J:215171 )

• in aged mice, the CD4/CD8 T cell ratio remains high in the spleen and lymph nodes unlike in wild-type mice in which the CD4/CD8 T cell ratio gradually decreases with age

increased CD4-positive, alpha-beta T cell number ( J:215171 )

• absolute number of CD4+ T cells is slightly increased

abnormal CD4-positive, alpha-beta T cell physiology ( J:215171 )

• CD4+ T cells show hyper-proliferation when stimulated with anti-CD3 antibody (TCR activation), but not when stimulated with PMA and ionomycin

• CD4+ T cells exhibit increased cell cycle progression following CD3 stimulation, with more T cells in S phase with a concomitant decrease in the corresponding cells in G0 phase

• CD4+ T cells produce lower levels of IL-2 upon stimulation with anti-CD3 antibody, indicating reduced effector function of CD4+ T cells, however this defect is diminished when cells are strongly stimulated with anti-CD3 and anti-CD28 agonists

hematopoietic system

increased T cell proliferation ( J:215171 )

• CD4+ T cells show hyper-proliferation when stimulated with anti-CD3 antibody (TCR activation), but not when stimulated with PMA and ionomycin

abnormal T cell subpopulation ratio ( J:215171 )

• in aged mice, the CD4/CD8 T cell ratio remains high in the spleen and lymph nodes unlike in wild-type mice in which the CD4/CD8 T cell ratio gradually decreases with age

increased CD4-positive, alpha-beta T cell number ( J:215171 )

• absolute number of CD4+ T cells is slightly increased

abnormal CD4-positive, alpha-beta T cell physiology ( J:215171 )

• CD4+ T cells show hyper-proliferation when stimulated with anti-CD3 antibody (TCR activation), but not when stimulated with PMA and ionomycin

• CD4+ T cells exhibit increased cell cycle progression following CD3 stimulation, with more T cells in S phase with a concomitant decrease in the corresponding cells in G0 phase

• CD4+ T cells produce lower levels of IL-2 upon stimulation with anti-CD3 antibody, indicating reduced effector function of CD4+ T cells, however this defect is diminished when cells are strongly stimulated with anti-CD3 and anti-CD28 agonists

cellular

increased T cell proliferation ( J:215171 )

• CD4+ T cells show hyper-proliferation when stimulated with anti-CD3 antibody (TCR activation), but not when stimulated with PMA and ionomycin

Genotype
MGI:5606171

cx2

• Allelic Composition: Tg(TcraTcrb)425Cbn/0; Trim30a^tm1Yjk/Trim30a^tm1Yjk
• Genetic Background: involves: 129P2/OlaHsd * BALB/c * C57BL/6

immune system

increased T cell proliferation ( J:215171 )

• T cells exhibit 10-20% enhanced proliferation compared to single Tg(TcraTcrb)425Cbn T cells, particularly when stimulated with a low dose of OVA(323-339) peptide

hematopoietic system

increased T cell proliferation ( J:215171 )

• T cells exhibit 10-20% enhanced proliferation compared to single Tg(TcraTcrb)425Cbn T cells, particularly when stimulated with a low dose of OVA(323-339) peptide

cellular

increased T cell proliferation ( J:215171 )

• T cells exhibit 10-20% enhanced proliferation compared to single Tg(TcraTcrb)425Cbn T cells, particularly when stimulated with a low dose of OVA(323-339) peptide