Phenotypes associated with this allele

• Allele Symbol: Mcrs1^{tm1.1(KOMP)Vlcg}
• Allele Name: targeted mutation 1.1, Velocigene
• Allele ID: MGI:5581465
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mcrs1^tm1.1(KOMP)Vlcg/Mcrs1^tm1.1(KOMP)Vlcg	B6N(Cg)-Mcrs1^tm1.1(KOMP)Vlcg/J	MGI:6377011
hm2	Mcrs1^tm1.1(KOMP)Vlcg/Mcrs1^tm1.1(KOMP)Vlcg	C57BL/6N-Mcrs1^tm1.1(KOMP)Vlcg/J	MGI:5757349
ht3	Mcrs1^tm1.1(KOMP)Vlcg/Mcrs1^+	C57BL/6N-Mcrs1^tm1.1(KOMP)Vlcg/J	MGI:5785080

Genotype
MGI:6377011

hm1

• Allelic Composition: Mcrs1^{tm1.1(KOMP)Vlcg}/Mcrs1^{tm1.1(KOMP)Vlcg}
• Genetic Background: B6N(Cg)-Mcrs1^{tm1.1(KOMP)Vlcg}/J
• Cell Lines: 16025A-A2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:279207 )

• although normal looking blastocysts are present at slightly higher than expected Mendelian ratios at E3.5 (29% versus 25%), no homozygous embryos or excess resorption sites are observed at E7.5

embryo

absent inner cell mass proliferation ( J:279207 )

• although blastocysts hatch from the zona pellucida with apparent trophectoderm (TE) and primitive endoderm (PrE) cells, inner cell mass (ICM) cells fail to form a typical outgrowth colony after 3 days in culture

decreased embryonic epiblast cell proliferation ( J:279207 )

• ICM/epiblast cells fail to form a typical outgrowth colony after 3 days in culture

cellular

absent inner cell mass proliferation ( J:279207 )

• although blastocysts hatch from the zona pellucida with apparent trophectoderm (TE) and primitive endoderm (PrE) cells, inner cell mass (ICM) cells fail to form a typical outgrowth colony after 3 days in culture

decreased embryonic epiblast cell proliferation ( J:279207 )

• ICM/epiblast cells fail to form a typical outgrowth colony after 3 days in culture

Genotype
MGI:5757349

hm2

• Allelic Composition: Mcrs1^{tm1.1(KOMP)Vlcg}/Mcrs1^{tm1.1(KOMP)Vlcg}
• Genetic Background: C57BL/6N-Mcrs1^{tm1.1(KOMP)Vlcg}/J
• Cell Lines: 16025A-A2

Data Sources

IMPC Data for Mcrs1

mortality/aging

embryonic lethality prior to organogenesis ( J:211773 )

IMPC - JAX

preweaning lethality, complete penetrance ( J:211773 )

IMPC - JAX

Genotype
MGI:5785080

ht3

• Allelic Composition: Mcrs1^{tm1.1(KOMP)Vlcg}/Mcrs1⁺
• Genetic Background: C57BL/6N-Mcrs1^{tm1.1(KOMP)Vlcg}/J
• Cell Lines: 16025A-A2

Data Sources

IMPC Data for Mcrs1

homeostasis/metabolism

increased circulating chloride level ( J:211773 )

♀

IMPC - JAX