All Phenotypes Prdm16<tm1.1Brsp> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Prdm16^tm1.1Brsp/Prdm16^tm1.1Brsp	B6.129-Prdm16^tm1.1Brsp	MGI:5564787
cn2	A1cf^{Tg(Myh6-cre/Esr1*)1Jmk}/A1cf⁺ Prdm16^tm1.1Brsp/Prdm16^tm1.1Brsp	B6.Cg-Prdm16^tm1.1Brsp A1cf^{Tg(Myh6-cre/Esr1*)1Jmk}	MGI:7314699
cn3	Prdm16^tm1.1Brsp/Prdm16^tm1.1Brsp Tg(Tnnt2-cre)5Blh/0	involves: 129 * C57BL/6 * C57BL/6J * DBA/2	MGI:7314697
cn4	Prdm16^tm1.1Brsp/Prdm16^tm1.1Brsp Tg(myl7.L-cre)1118Tmhn/0	involves: 129 * C57BL/6J * MF1	MGI:7314277
cn5	Prdm16^tm1.1Brsp/Prdm16^tm1.1Brsp Myf5^tm1(cre)Mrc/Myf5⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:6107172
cn6	Mecom^tm1Miku/Mecom^tm1Miku Prdm16^tm1.1Brsp/Prdm16^tm1.1Brsp Myf5^tm1(cre)Mrc/Myf5⁺	involves: 129S1/Sv * 129X1/SvJ * C57BL/6NCrlj * CBA/JCrlj	MGI:6107171
cn7	Prdm16^tm1.1Brsp/Prdm16^tm1.1Brsp Tg(Adipoq-cre)1Evdr/0	involves: 129/Sv * C57BL/6 * FVB/N	MGI:5564786

Allelic Composition	Prdm16^tm1.1Brsp/Prdm16^tm1.1Brsp
Genetic Background	B6.129-Prdm16^tm1.1Brsp

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prdm16^tm1.1Brsp mutation (1 available); any Prdm16 mutation (72 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

normal phenotype

no abnormal phenotype detected ( J:208683 )

• mice are viable and fertile

Allelic Composition	A1cf^{Tg(Myh6-cre/Esr1*)1Jmk}/A1cf⁺ Prdm16^tm1.1Brsp/Prdm16^tm1.1Brsp
Genetic Background	B6.Cg-Prdm16^tm1.1Brsp A1cf^{Tg(Myh6-cre/Esr1*)1Jmk}

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
A1cf^{Tg(Myh6-cre/Esr1*)1Jmk} mutation (5 available); any A1cf mutation (39 available)
Prdm16^tm1.1Brsp mutation (1 available); any Prdm16 mutation (72 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cardiovascular system

cardiovascular system phenotype ( J:326525 )

N	• tamoxifen-treated mice do not show overt cardiac phenotypes, even 36 weeks after induction

Allelic Composition	Prdm16^tm1.1Brsp/Prdm16^tm1.1Brsp Tg(Tnnt2-cre)5Blh/0
Genetic Background	involves: 129 * C57BL/6 * C57BL/6J * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prdm16^tm1.1Brsp mutation (1 available); any Prdm16 mutation (72 available)
Tg(Tnnt2-cre)5Blh mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cardiovascular system

dilated heart left ventricle ( J:326525 )

mortality/aging

postnatal lethality, complete penetrance ( J:326525 )

• postnatal lethality before P7

Allelic Composition	Prdm16^tm1.1Brsp/Prdm16^tm1.1Brsp Tg(myl7.L-cre)1118Tmhn/0
Genetic Background	involves: 129 * C57BL/6J * MF1

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prdm16^tm1.1Brsp mutation (1 available); any Prdm16 mutation (72 available)
Tg(myl7.L-cre)1118Tmhn mutation (2 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

postnatal lethality, complete penetrance ( J:326525 )

• mice exhibit progressive cardiac dysfunction leading to death before P7

cardiovascular system

thin left ventricle myocardium compact layer ( J:326525 )

• thinner left ventricle compact myocardium starting from E15.5

• however, thickness of right ventricle compact myocardium is normal

abnormal heart development ( J:326525 )

• mice develop biventricular noncompaction

abnormal heart apex morphology ( J:326525 )

• mice exhibit clefts at the apices of the hearts as early as E15.5

thin interventricular septum ( J:326525 )

• thinner interventricular septum starting from E15.5

• however, thickness of right ventricle compact myocardium is not different

increased heart left ventricle size ( J:326525 )

• enlarged left ventricle is seen as early as E15.5

dilated heart left ventricle ( J:326525 )

abnormal cardiovascular system physiology ( J:326525 )

• mice exhibit progressive cardiac dysfunction

decreased cardiac muscle contractility ( J:326525 )

• severe cardiac contractile dysfunction in P0 to P3 neonates

decreased fetal cardiomyocyte proliferation ( J:326525 )

• reduction in proliferating cardiomyocytes in left ventricle compact myocardium and interventricular septum at both E13.5 and E15.5

• however, no differences in cardiomyocyte apoptosis are seen

abnormal heart echocardiography feature ( J:326525 )

• echocardiography shows severe cardiac contractile dysfunction in P0 to P3 neonates, increased left ventricle chamber size, and thinned interventricular septum

cardiomyopathy ( J:326525 )

• left ventricular noncompaction cardiomyopathy

muscle

thin left ventricle myocardium compact layer ( J:326525 )

• thinner left ventricle compact myocardium starting from E15.5

• however, thickness of right ventricle compact myocardium is normal

decreased cardiac muscle contractility ( J:326525 )

• severe cardiac contractile dysfunction in P0 to P3 neonates

decreased fetal cardiomyocyte proliferation ( J:326525 )

• reduction in proliferating cardiomyocytes in left ventricle compact myocardium and interventricular septum at both E13.5 and E15.5

• however, no differences in cardiomyocyte apoptosis are seen

cardiomyopathy ( J:326525 )

• left ventricular noncompaction cardiomyopathy

cellular

decreased fetal cardiomyocyte proliferation ( J:326525 )

• reduction in proliferating cardiomyocytes in left ventricle compact myocardium and interventricular septum at both E13.5 and E15.5

• however, no differences in cardiomyocyte apoptosis are seen

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
left ventricular noncompaction		DOID:0060480	OMIM:604169	J:326525

Allelic Composition	Prdm16^tm1.1Brsp/Prdm16^tm1.1Brsp Myf5^tm1(cre)Mrc/Myf5⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Myf5^tm1(cre)Mrc mutation (1 available); any Myf5 mutation (17 available)
Prdm16^tm1.1Brsp mutation (1 available); any Prdm16 mutation (72 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

adipose tissue

decreased body fat mass ( J:213153 )

abnormal brown adipose tissue morphology ( J:213153 )

• pale axillary and cervical brown adipose tissue depots

• however, tissue mass is not altered

increased brown fat cell lipid droplet size ( J:213153 )

• in the interscapular brown adipose tissue of mice older than 6 months

decreased brown fat lipid droplet number ( J:213153 )

• in the interscapular brown adipose tissue of mice older than 6 months

whitened brown adipose tissue morphology ( J:213153 )

• whitening in mice older than 6 months with increased size of the tissue, a switch from multilocular to unilocular morphology, and increased lipid content

decreased fat cell mitochondrial DNA content ( J:213153 )

• in unstimulated interscapular brown adipose tissue

abnormal interscapular fat pad morphology ( J:213153 )

• whitening in mice older than 6 months with increased size of the tissue, a switch from multilocular to unilocular morphology, and increased lipid content

• 3 week old mice fed a high-fat diet exhibit loss of normal brown adipocyte morphology unlike wild-type mice

increased interscapular fat pad weight ( J:213153 )

• in mice older than 6 months

• however, juvenile mice exhibit normal sized brown adipose tissue depots

abnormal brown adipose tissue thermogenesis ( J:213153 )

• precipitous drop in body temperature following exposure to cold

• however, diet-induced thermogenesis is normal

homeostasis/metabolism

impaired adaptive thermogenesis ( J:213153 )

• precipitous drop in body temperature following exposure to cold

abnormal oxygen consumption ( J:213153 )

improved glucose tolerance ( J:213153 )

growth/size/body

decreased body fat mass ( J:213153 )

decreased lean body mass ( J:213153 )

decreased body weight ( J:213153 )

• at all ages

decreased body length ( J:213153 )

cellular

decreased fat cell mitochondrial DNA content ( J:213153 )

• in unstimulated interscapular brown adipose tissue

decreased mitochondrial number ( J:213153 )

• with poorly organized cristae in unstimulated interscapular brown adipose tissue

abnormal cellular respiration ( J:213153 )

• reduced basal in interscapular brown adipose tissue

Allelic Composition	Mecom^tm1Miku/Mecom^tm1Miku Prdm16^tm1.1Brsp/Prdm16^tm1.1Brsp Myf5^tm1(cre)Mrc/Myf5⁺
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6NCrlj * CBA/JCrlj

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mecom^tm1Miku mutation (0 available); any Mecom mutation (81 available)
Myf5^tm1(cre)Mrc mutation (1 available); any Myf5 mutation (17 available)
Prdm16^tm1.1Brsp mutation (1 available); any Prdm16 mutation (72 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

adipose tissue

increased brown fat cell lipid droplet size ( J:213153 )

• in the interscapular brown adipose tissue of mice older than 6 months

abnormal interscapular fat pad morphology ( J:213153 )

• pale in 3 month old mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

adipose tissue

abnormal adipose tissue morphology ( J:208683 )

• subcutaneous adipose tissue contains larger adipocytes and fewer smaller multiocular UCP1+ adipocytes relative to control mice following cold exposure

• subcutaneous adipose tissue from mice following 6 weeks on high fat diet has increased numbers of crown like structures, however, there are no changes in visceral (abdominal) fat

• subcutaneous adipose tisse from mice following 6 weeks on high fat diet has increased numbers of CD11b+F4/80+ macrophages

increased subcutaneous adipose tissue amount ( J:208683 )

• subcutaneous fat mass from male mice after 16 weeks on high fat diet is increased to twice that of controls, epididymal and brown adipose tissue are unchanged

increased total body fat amount ( J:208683 )

• body composition analysis after 16 weeks on high fat diet indicates increased fat mass with no change in lean body mass

abnormal adipose tissue distribution ( J:208683 )

• subcutaneous fat mass is doubled as compared to controls after 16 weeks on high fat diet; epididymal and BAT masses are unchanged

increased fat cell size ( J:208683 )

• subcutaneous adipocytes from male mice after 18 weeks on high fat diet exhibit a 33% increase in mean adipocyte area

abnormal adipose tissue physiology ( J:208683 )

• O₂ consumption is reduced in subcutaneous adipose pads, but not in brown adipose pads, at baseline (36% reduced) and following stimulation with a beta-adrenergic agonist (49% reduced)

decreased adipocyte glucose uptake ( J:208683 )

• glucose uptake is reduced in subcutaneous fat (79% decrease) and visceral fat (53% decrease) after 6 weeks on high fat diet as compared to controls

abnormal brown adipose tissue thermogenesis ( J:208683 )

• subcutaneous adipocytes exhibit a blunted response to stimuli such as isoproterenol that induce a thermogenic gene program (beige adipocytes)

growth/size/body

increased susceptibility to weight gain ( J:208683 )

• mice exhibit increased weight gain after 16 weeks on high-fat, high-carbohydrate diet relative to control mice

hematopoietic system

increased macrophage cell number ( J:208683 )

• subcutaneous adipose tissue from mice following 6 weeks on high fat diet has increased numbers of CD11b+F4/80+ macrophages, but macrophage numbers in visceral adipose tissue and spleen are unchanged

homeostasis/metabolism

increased circulating insulin level ( J:208683 )

• increased fasting plasma insulin levels (55%) in mice after 6 weeks on high fat diet

abnormal oxygen consumption ( J:208683 )

• unlike controls, mutant mice do not exhibit increased O₂ consumption following stimulation with a beta-adrenergic agonist

increased respiratory quotient ( J:208683 )

• mice exhibit an increased respiratory exchange ratio (RER), but no increase in O₂ consumption

insulin resistance ( J:208683 )

• a reduced glucose infusion rate (64% of controls) is observed in mice after 6 weeks on high fat diet

• decreased whole body glucose uptake is observed in mice after 6 weeks on high fat diet

• clamped insulin levels do not increase as much as controls in mice after 6 weeks on high fat diet

increased liver triglyceride level ( J:208683 )

• increased liver tryglycerides are observed in mice after 6 weeks on high fat diet as compared to controls

immune system

increased macrophage cell number ( J:208683 )

integument

increased subcutaneous adipose tissue amount ( J:208683 )

• subcutaneous fat mass from male mice after 16 weeks on high fat diet is increased to twice that of controls, epididymal and brown adipose tissue are unchanged

liver/biliary system

increased liver triglyceride level ( J:208683 )

• increased liver tryglycerides are observed in mice after 6 weeks on high fat diet as compared to controls

hepatic steatosis ( J:208683 )

• after 6 weeks on high fat diet

cellular

decreased adipocyte glucose uptake ( J:208683 )

• glucose uptake is reduced in subcutaneous fat (79% decrease) and visceral fat (53% decrease) after 6 weeks on high fat diet as compared to controls

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