Phenotypes associated with this allele

• Allele Symbol: Bub1b^tm1.1Hsl
• Allele Name: targeted mutation 1.1, Hyunsook Lee
• Allele ID: MGI:5543300
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Bub1b^tm1.1Hsl/Bub1b^tm1.1Hsl	involves: 129 * C57BL/6	MGI:5543390
ht2	Bub1b^tm1.1Hsl/Bub1b^+	involves: 129 * C57BL/6	MGI:5543391
cx3	Bub1b^tm1.1Hsl/Bub1b^+ Tg(HIST1H2BB/EGFP)1Pa/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5543392

Genotype
MGI:5543390

hm1

• Allelic Composition: Bub1b^tm1.1Hsl/Bub1b^tm1.1Hsl
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between implantation and somite formation, complete penetrance ( J:201641 )

• mice die at approximately E6.5

embryo

abnormal embryo development ( J:201641 )

• 4 of 17 E6.5 mice exhibit abnormal morphology with massive TUNEL staining

embryonic growth retardation ( J:201641 )

• of some cultured E3.5 embryos

cellular

increased apoptosis ( J:201641 )

• 4 of 17 E6.5 mice exhibit abnormal morphology with massive TUNEL staining

growth/size/body

embryonic growth retardation ( J:201641 )

• of some cultured E3.5 embryos

Genotype
MGI:5543391

ht2

• Allelic Composition: Bub1b^tm1.1Hsl/Bub1b⁺
• Genetic Background: involves: 129 * C57BL/6

Spontaneous tumorigenesis in Bub1b^tm1.1Hsl/Bub1b⁺ mice

digestive/alimentary system

increased rectum adenocarcinoma incidence ( J:201641 )

• in 1 of 121 mice

mortality/aging

mortality/aging ( J:201641 )

N • mice are born in Mendelian ratios and do not display premature aging unlike Bub1b^tm2.1Jvd heterozygotes

liver/biliary system

increased hepatocellular carcinoma incidence ( J:201641 )

• in 6 of 121 mice

neoplasm

increased hepatocellular carcinoma incidence ( J:201641 )

• in 6 of 121 mice

increased leukemia incidence ( J:201641 )

• megakaryocytic leukemia in 1 of 121 mice

• metastatic lymphoma/leukemia in 3 of 121 mice

increased lymphoma incidence ( J:201641 )

• metastatic lymphoma/leukemia in 3 of 121 mice

increased B cell derived lymphoma incidence ( J:201641 )

• in 11 of 121 mice

increased adenocarcinoma incidence ( J:201641 )

• in 2 of 121 mice (including 1 mouse with lung adenocarcinoma and 1 mouse with rectal adenocarcinoma)

increased rectum adenocarcinoma incidence ( J:201641 )

• in 1 of 121 mice

increased lung adenocarcinoma incidence ( J:201641 )

• in 1 of 121 mice

increased sarcoma incidence ( J:201641 )

• in 4 of 121 mice (including 2 mice with smooth muscle sarcoma, 1 mouse with endometrial stromal sarcoma and 1 mouse with angiosarcoma)

increased fibrosarcoma incidence ( J:201641 )

• in 1 of 121 mice

increased hemangiosarcoma incidence ( J:201641 )

• in 1 of 121 mice

increased incidence of induced tumors ( J:201641 )

• both solid and hematologic tumors in 46 of 121 mice (including malignant tumors in 28 of 121 mice and benign tumors in 18 of 121 mice)

immune system

increased B cell proliferation ( J:201641 )

• in mice with B cell lymphoma

enlarged spleen ( J:201641 )

increased spleen white pulp amount ( J:201641 )

• in mice with B cell lymphoma

respiratory system

increased lung adenocarcinoma incidence ( J:201641 )

• in 1 of 121 mice

hematopoietic system

increased B cell proliferation ( J:201641 )

• in mice with B cell lymphoma

enlarged spleen ( J:201641 )

increased spleen white pulp amount ( J:201641 )

• in mice with B cell lymphoma

cellular

increased B cell proliferation ( J:201641 )

• in mice with B cell lymphoma

growth/size/body

enlarged spleen ( J:201641 )

Genotype
MGI:5543392

cx3

• Allelic Composition: Bub1b^tm1.1Hsl/Bub1b⁺; Tg(HIST1H2BB/EGFP)1Pa/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

cellular

aneuploidy ( J:201641 )

• in mouse embryonic fibroblasts

increased micronucleus incidence ( J:201641 )

• micronuclei due to lagging chromosomes in mouse embryonic fibroblasts, regenerating hepatocytes and hepatocellular carcinoma

abnormal mitosis ( J:201641 )

• premature sister chromatid separation in mouse embryonic fibroblasts

• shortened mitotic timing

abnormal mitotic spindle assembly checkpoint ( J:201641 )

• weakened with chromosome mis-segregation, congression failure and severe defects in kinetochore-microtubule attachment in mouse embryonic fibroblasts