Phenotypes associated with this allele

• Allele Symbol: Gabrd^tm1.1Jmag
• Allele Name: targeted mutation 1.1, Jamie Maguire
• Allele ID: MGI:5526859
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gabrd^tm1.1Jmag/Gabrd^+ Gad2^tm2(cre)Zjh/Gad2^+	involves: 129S4/SvJae * C57BL/6 * SJL	MGI:5527106

Genotype
MGI:5527106

cn1

• Allelic Composition: Gabrd^tm1.1Jmag/Gabrd⁺; Gad2^tm2(cre)Zjh/Gad2⁺
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

decreased susceptibility to pharmacologically induced seizures ( J:203336 )

• decreased seizure susceptibility in response 20mg/kg kainic acid as compared to controls

• decreased epilepitform activity over 120 min recording period

impaired ability to fire action potentials ( J:203336 )

• decreased average number of action potentials fired in CA1 pyramidal neurons in response to current injections above 40 pA

• decreased average number of action potentials fired in dentate gyrus granule cells in response to current injections above 60 pA

abnormal GABAergic neuron physiology ( J:203336 )

• decrease in tonic GABAergic currents recorded in interneurons from the molecular layer of the hippocampus as compared to controls

• decrease in tonic GABAergic currents recorded in interneurons from the stratum radiatum layer of the hippocampus as compared to controls

• increase in tonic GABAergic currents recorded in CA1 pyramidal neurons, however, no difference is observed in dentate gyrus granule cells as compared to controls

abnormal CNS synaptic transmission ( J:203336 )

• increase in spontaneous firing rate of stratum radiatum layer interneurons as compared to controls

• increase in firing rate of stratum radiatum layer interneurons in response to a single 1 s, 100 pA current injection as compared to controls

• increase in firing rate of molecular layer interneurons in response to a single 1 s, 100 pA current injection as compared to controls

• no differences are observed in resting potential or input resistance

increased excitatory postsynaptic current amplitude ( J:203336 )

• half maximal current amplitude (I₅₀) is increased in CA1 pyramidal neurons and dentate gyrus granule cells as compared to controls

abnormal inhibitory postsynaptic currents ( J:203336 )

• frequency of spontaneous IPSCs is increased in dentate gyrus granule cells (DGGCs) and CA1 pyramidal neurons

• peak amplitude of spontaneous IPSCs is increased in DGGCs and CA1 pyramidal neurons

• decrease in average inter-event interval (IEI) of spontaneous IPSCs

• increase in percentage of DGGCs and CA1 pyramidal cells following bursts of inhibitory postsynaptic currents as compared to control

behavior/neurological

decreased susceptibility to pharmacologically induced seizures ( J:203336 )

• decreased seizure susceptibility in response 20mg/kg kainic acid as compared to controls

• decreased epilepitform activity over 120 min recording period