Analysis Tools
reproductive system
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• no Ddx4+ germ cells in ovaries at age 8 weeks
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• fewer spermatozoa-filled tubules in caput and caudal epididymis
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• misshapen sperm heads
• normal axoneme and flagellum morphology
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• misshapen sperm heads
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• failure in manchette disassembly during spermiogenesis steps 13-14
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• 50% reduction
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• 2.4x increase in percentage of stage XII tubules at age 7 months
• loss of round spermatids (61% reduction at stage III, 73% at stage VII) and elongated spermatids (71% at stage III, 82% at stage VII and 79% at stage XII)
• normal number of primary spermatocytes and spermatocytes in metaphase at stage III, VII and XII seminiferous tubules at age 7 months
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small testis
(
J:306320
)
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• at ages P28 and 7 months
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• at ages P28 and 7 months
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• meiosis initiation delay at ages P28 and adult
• G2/M defect at ages P28 and adult
• fewer spermatocytes in anaphase at ages P28 and adult
• mitotic spindle misalignment in adult metaphase spermatocytes
• under-duplication of centrioles in adult pachytene and diplotene spermatocytes with 75% containing 0-3 centrioles instead of 4
• normal prophase 1 at ages P28 and adult
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• no offspring from matings of mutant females with wild-type or mutant males
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• no offspring from matings of mutant males with wild-type or mutant females
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endocrine/exocrine glands
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• 2.4x increase in percentage of stage XII tubules at age 7 months
• loss of round spermatids (61% reduction at stage III, 73% at stage VII) and elongated spermatids (71% at stage III, 82% at stage VII and 79% at stage XII)
• normal number of primary spermatocytes and spermatocytes in metaphase at stage III, VII and XII seminiferous tubules at age 7 months
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small testis
(
J:306320
)
|
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• at ages P28 and 7 months
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• at ages P28 and 7 months
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cellular
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• no Ddx4+ germ cells in ovaries at age 8 weeks
|
|
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• fewer spermatozoa-filled tubules in caput and caudal epididymis
|
|
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• misshapen sperm heads
• normal axoneme and flagellum morphology
|
|
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• misshapen sperm heads
|
|
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• failure in manchette disassembly during spermiogenesis steps 13-14
|
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• 50% reduction
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nervous system
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• decreased number of Pax6+ apical progenitors in E14.5 embryos
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• 10% increase in differentiating cells in E16.5 embryos
• normal apoptosis level in E14.5 embryos
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• reduced thickness of upper layers II-IV in newborns (age P0)
• reduced upper layers II-IV to lower layers V-VI neuron count in newborns
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growth/size/body
| N |
• normal growth in first 12 weeks
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