Phenotypes associated with this allele

• Allele Symbol: Rraga^tm1.1Dmsa
• Allele Name: targeted mutation 1.1, David Sabatini
• Allele ID: MGI:5499905
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rraga^tm1.1Dmsa/Rraga^tm1.1Dmsa	involves: 129 * C57BL/6 * C57BL/6J	MGI:5499979

Genotype
MGI:5499979

hm1

• Allelic Composition: Rraga^tm1.1Dmsa/Rraga^tm1.1Dmsa
• Genetic Background: involves: 129 * C57BL/6 * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

increased susceptibility to induced morbidity/mortality ( J:194401 )

• in fasted neonates

• however, treatment with rapamycin, glucose or a mix of gluconeogenic amino acids partially rescues accelerated lethality

neonatal lethality, complete penetrance ( J:194401 )

• mice die within the first day after birth

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:194401 )

N • mice exhibit normal levels of lactate

abnormal autophagy ( J:194401 )

• autophagic vacuoles are rarely observed in the liver or skeletal muscle in fasted mice unlike in wild-type mice

abnormal circulating amino acid level ( J:194401 )

• decreased in neonates

• however, rapamycin treatment restores amino acid levels

hypoglycemia ( J:194401 )

• in fasted neonates that does not recover

• increased in fasted mice treated with alanine

abnormal gluconeogenesis ( J:194401 )

• reduced in the liver

abnormal glycogen homeostasis ( J:194401 )

• fasted mice exhibit accelerated hepatic glycogen use compared with wild-type mice

• however, mice exhibit normal hepatic glycogen levels and rapamycin administration partly restored hepatic glycogen in fasted mice

growth/size/body

decreased birth weight ( J:194401 )

• in mice delivered by Caesarian section

cellular

abnormal autophagy ( J:194401 )

• autophagic vacuoles are rarely observed in the liver or skeletal muscle in fasted mice unlike in wild-type mice