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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Cbx4tm1.2Gxu
targeted mutation 1.2, Guo-Liang Xu
MGI:5489932
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Cbx4tm1.2Gxu/Cbx4tm1.2Gxu involves: 129S2/SvPas * C57BL/6J MGI:5489933
cn2
 		Cbx4tm1.1Gxu/Cbx4tm1.2Gxu
Tg(Foxn1-cre)1Tbo/0 		involves: 129S2/SvPas * C57BL/6J MGI:5489934


Genotype
MGI:5489933
hm1
Allelic
Composition		 Cbx4tm1.2Gxu/Cbx4tm1.2Gxu
Genetic
Background		 involves: 129S2/SvPas * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cbx4tm1.2Gxu mutation (0 available); any Cbx4 mutation (16 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
neonatal lethality, complete penetrance ( J:194053 )
• die within 1 hr of birth

hematopoietic system
hematopoietic system phenotype ( J:194053 )
N
• total numbers of splenocytes and bone marrow cells are similar to wild-type littermates
abnormal thymus development ( J:194053 )
• growth of the thymus is severely retarded after E13.5
• impaired proliferation of thymocytes at E17.5
thymus hypoplasia ( J:194053 )
• thymic hypogenesis, with a decrease in both total thymic cells and thymic epithelial cells
• however, the ratios of the different cell types are similar to controls
abnormal thymus physiology ( J:194053 )
• impaired proliferation of thymocytes at E17.5
• defect in proliferation is due to defect in the stroma, as reconstituted fetal thymic organ cultures using mutant thymi but wild-type hematopoietic progenitor cells show barely detectable numbers of thymocytes

immune system
abnormal thymus development ( J:194053 )
• growth of the thymus is severely retarded after E13.5
• impaired proliferation of thymocytes at E17.5
thymus hypoplasia ( J:194053 )
• thymic hypogenesis, with a decrease in both total thymic cells and thymic epithelial cells
• however, the ratios of the different cell types are similar to controls
abnormal thymus physiology ( J:194053 )
• impaired proliferation of thymocytes at E17.5
• defect in proliferation is due to defect in the stroma, as reconstituted fetal thymic organ cultures using mutant thymi but wild-type hematopoietic progenitor cells show barely detectable numbers of thymocytes

endocrine/exocrine glands
abnormal thymus development ( J:194053 )
• growth of the thymus is severely retarded after E13.5
• impaired proliferation of thymocytes at E17.5
thymus hypoplasia ( J:194053 )
• thymic hypogenesis, with a decrease in both total thymic cells and thymic epithelial cells
• however, the ratios of the different cell types are similar to controls
abnormal thymus physiology ( J:194053 )
• impaired proliferation of thymocytes at E17.5
• defect in proliferation is due to defect in the stroma, as reconstituted fetal thymic organ cultures using mutant thymi but wild-type hematopoietic progenitor cells show barely detectable numbers of thymocytes




Genotype
MGI:5489934
cn2
Allelic
Composition		 Cbx4tm1.1Gxu/Cbx4tm1.2Gxu
Tg(Foxn1-cre)1Tbo/0
Genetic
Background		 involves: 129S2/SvPas * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cbx4tm1.1Gxu mutation (0 available); any Cbx4 mutation (16 available)
Cbx4tm1.2Gxu mutation (0 available); any Cbx4 mutation (16 available)
Tg(Foxn1-cre)1Tbo mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal thymus development ( J:194053 )
• expression analysis indicates a defect in the maturation of both medullary and cortical epithelial cells
thymus atrophy ( J:194053 )
• limited thymic epithelial architecture quickly deteriorates in postnatal mice with discrete cortical and medullary structures no longer seen at 3 weeks of age
abnormal thymus physiology ( J:194053 )
• expression analysis indicates impaired proliferation of developing epithelial cells
abnormal lymphopoiesis ( J:194053 )
• the double negative population is over-represented and composed primarily of B220+ B lineage cells instead of T lineage precursors
• however, the absolute number of B lineage cells in the thymus is similar to controls
decreased T cell number ( J:194053 )
• the T cell compartment in the periphery is never fully established in adult mice
• the CD3+ population in the spleen is small
decreased double-positive T cell number ( J:194053 )
• markedly reduced in proportion in the thymus at 3 weeks of age
• barely detectable at 6 weeks of age

immune system
abnormal thymus development ( J:194053 )
• expression analysis indicates a defect in the maturation of both medullary and cortical epithelial cells
thymus atrophy ( J:194053 )
• limited thymic epithelial architecture quickly deteriorates in postnatal mice with discrete cortical and medullary structures no longer seen at 3 weeks of age
abnormal thymus physiology ( J:194053 )
• expression analysis indicates impaired proliferation of developing epithelial cells
abnormal lymphopoiesis ( J:194053 )
• the double negative population is over-represented and composed primarily of B220+ B lineage cells instead of T lineage precursors
• however, the absolute number of B lineage cells in the thymus is similar to controls
decreased double-positive T cell number ( J:194053 )
• markedly reduced in proportion in the thymus at 3 weeks of age
• barely detectable at 6 weeks of age
decreased T cell number ( J:194053 )
• the T cell compartment in the periphery is never fully established in adult mice
• the CD3+ population in the spleen is small

endocrine/exocrine glands
abnormal thymus development ( J:194053 )
• expression analysis indicates a defect in the maturation of both medullary and cortical epithelial cells
thymus atrophy ( J:194053 )
• limited thymic epithelial architecture quickly deteriorates in postnatal mice with discrete cortical and medullary structures no longer seen at 3 weeks of age
abnormal thymus physiology ( J:194053 )
• expression analysis indicates impaired proliferation of developing epithelial cells




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Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory