Phenotypes associated with this allele

• Allele Symbol: Hdc^{tm1.1(icre)Wwis}
• Allele Name: targeted mutation 1.1, William Wisden
• Allele ID: MGI:5461698
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gabrg2^tm1Wul/Gabrg2^tm1Wul Hdc^tm1.1(icre)Wwis/Hdc^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:5461821
cn2	Gabbr1^tm2Bet/Gabbr1^tm2Bet Hdc^tm1.1(icre)Wwis/Hdc^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6J	MGI:5461822

Genotype
MGI:5461821

cn1

• Allelic Composition: Gabrg2^tm1Wul/Gabrg2^tm1Wul; Hdc^{tm1.1(icre)Wwis}/Hdc⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal neuron physiology ( J:188103 )

• tuberomammillary nucleus (TMN) neurons lacking GABAA receptors are more excitable than control neurons; more action potentials (APs) are produced for a given current injection and current needed to reach threshold for AP generation is reduced significantly

• tuberomammillary nucleus neurons have increased membrane time constants compared to control neurons

abnormal GABA-mediated receptor currents ( J:188103 )

• whole cell voltage-clamped mutant neurons in the ventral tuberomammillary (VTM) nucleus lack fast GABAA receptor-mediated IPSCs, but are IPSCs are observed in controls

• in dorsal lateral geniculate (DLG) nucleus slice preparations, only 30% of neurons exhibit GABAergic IPSCs compared to most neurons tested in control slices

abnormal inhibitory postsynaptic currents ( J:188103 )

behavior/neurological

behavior/neurological phenotype ( J:188103 )

N • mice appear indistinguishable from controls in health, behavior and appearance

N • total sleep-wake time, EEG power spectrum, and duration of REM and non-REM (NREM) periods during the 24 hour cycle do not differ from controls

N • habituation to a new environment is not different from controls

N • propofol-induced loss of righting reflex (LORR) is not different from controls

Genotype
MGI:5461822

cn2

• Allelic Composition: Gabbr1^tm2Bet/Gabbr1^tm2Bet; Hdc^{tm1.1(icre)Wwis}/Hdc⁺
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J

behavior/neurological

behavior/neurological phenotype ( J:188103 )

N • mice appear indistinguishable from controls in health, behavior and appearance

N • EEG or sleep-wake parameters during the 24 hour cycle do not differ from controls

N • propofol-induced loss of righting reflex (LORR) is not different from controls

abnormal habituation to a new environment ( J:188103 )

• level of arousal (time spent in the wake phase) remains elevated for a longer period in mutants in response to a change of environment, compared to controls

nervous system

abnormal neuron physiology ( J:188103 )

• tuberomammillary nucleus neurons in brain slices from mutant animals are not hyperpolarized by propofol treatment, as observed in control neurons