Phenotypes associated with this allele

• Allele Symbol: Rspo3^tm1.2Jcob
• Allele Name: targeted mutation 1.2, John Cobb
• Allele ID: MGI:5443965
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rspo3^tm1.2Jcob/Rspo3^tm1.2Jcob	involves: 129S6/SvEvTac * C57BL/6 * CD-1 * SJL	MGI:5443988
cn2	Rspo3^tm1.1Jcob/Rspo3^tm1.2Jcob Tg(Prrx1-cre)1Cjt/0	involves: 129S6/SvEvTac * C57BL/6 * CD-1 * SJL	MGI:5443989
cn3	Rspo2^ftls/Rspo2^ftls Rspo3^tm1.1Jcob/Rspo3^tm1.2Jcob Tg(Prrx1-cre)1Cjt/0	involves: 129S6/SvEvTac * C57BL/6 * CD-1 * SJL	MGI:5443990

Genotype
MGI:5443988

hm1

• Allelic Composition: Rspo3^tm1.2Jcob/Rspo3^tm1.2Jcob
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * CD-1 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Developmental arrest and vascular defects in Rspo3^tm1.2Jcob/Rspo3^tm1.2Jcob embryos

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:188812 )

• mice die around E10

embryo

embryonic growth arrest ( J:188812 )

• development does not progress beyond early E10

decreased embryo size ( J:188812 )

• at E10.5 to E10.75

pale yolk sac ( J:188812 )

• at E10.5 to E10.75

cardiovascular system

enlarged pericardium ( J:188812 )

• at E10.5 to E10.75

hemorrhage ( J:188812 )

• at E10.5 to E10.75

growth/size/body

decreased embryo size ( J:188812 )

• at E10.5 to E10.75

Genotype
MGI:5443989

cn2

• Allelic Composition: Rspo3^tm1.1Jcob/Rspo3^tm1.2Jcob; Tg(Prrx1-cre)1Cjt/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * CD-1 * SJL

Rspo2^ftls/Rspo2^ftls Rspo3^tm1.1Jcob/Rspo3^tm1.2Jcob Tg(Prrx1-cre)1Cjt/0 double mutants have more severe limb defects than single mutants

limbs/digits/tail

short limbs ( J:188812 )

• slight in newborns

• however, adult mice exhibit normal limb length

Genotype
MGI:5443990

cn3

• Allelic Composition: Rspo2^ftls/Rspo2^ftls; Rspo3^tm1.1Jcob/Rspo3^tm1.2Jcob; Tg(Prrx1-cre)1Cjt/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * CD-1 * SJL

Rspo2^ftls/Rspo2^ftls Rspo3^tm1.1Jcob/Rspo3^tm1.2Jcob Tg(Prrx1-cre)1Cjt/0 double mutants have more severe limb defects than single mutants

mortality/aging

neonatal lethality, complete penetrance ( J:188812 )

• mice die at birth as do Rspo2^ftls homozygotes

limbs/digits/tail

abnormal digit morphology ( J:188812 )

• in the forelimb

adactyly ( J:188812 )

• in digits of the forelimbs in 5 of 6 mice; all mice lack 1 digit

brachydactyly ( J:188812 )

• three shortened middle digits

abnormal forelimb morphology ( J:188812 )

• more subtle than in hindlimbs

abnormal humerus morphology ( J:188812 )

abnormal hindlimb morphology ( J:188812 )

• at birth, mice lack most of their hindlimbs

• hindlimb abnormalities are more severe more proximally than in Rspo2^ftls homozygotes

bowed femur ( J:188812 )

• severely shortened and bent in two mice

short femur ( J:188812 )

• severely shortened in two mice

absent tibia ( J:188812 )

• in one mouse

short tibia ( J:188812 )

respiratory system

abnormal lung morphology ( J:188812 )

• as in Rspo2^ftls homozygotes

behavior/neurological

absent gastric milk in neonates ( J:188812 )

• as in Rspo2^ftls homozygotes

skeleton

abnormal humerus morphology ( J:188812 )

bowed femur ( J:188812 )

• severely shortened and bent in two mice

short femur ( J:188812 )

• severely shortened in two mice

absent tibia ( J:188812 )

• in one mouse

short tibia ( J:188812 )

abnormal scapula morphology ( J:188812 )