Phenotypes associated with this allele

• Allele Symbol: Tg(Wt1-cre)#Jbeb
• Allele Name: transgene insertion, John Burch
• Allele ID: MGI:5308608
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Notch1^tm1Agt/Notch1^tm1Agt Tg(Wt1-cre)#Jbeb/0	involves: 129	MGI:5316086
cn2	Gt(ROSA)26Sor^tm1(Notch1)Dam/Gt(ROSA)26Sor^+ Tg(Wt1-cre)#Jbeb/0	involves: 129S4/SvJaeSor	MGI:5316087

Genotype
MGI:5316086

cn1

• Allelic Composition: Notch1^tm1Agt/Notch1^tm1Agt; Tg(Wt1-cre)#Jbeb/0
• Genetic Background: involves: 129

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis ( J:178290 )

• embryos start to die shortly after E13.5

cardiovascular system

thin myocardium compact layer ( J:178290 )

• compact myocardium thickness is signficantly reduced in mutants

• number of nuclei is lower (25-30%) at E13.5, with reduction in proliferating cells observed compared to control

myocardium hypoplasia ( J:178290 )

• reduction in number of smooth muscle cells in the compact myocardium is observed at E13.5

abnormal coronary vessel morphology ( J:178290 )

• at E13.5 hearts exhibit severe reduction and disorganization of the coronary vascular plexus

• abnormal development of CVs is associated with endocardial-subepicardial connections that result in cysts with endothelial lining connected to the outer ventricular myocardium

absent coronary vessels ( J:178290 )

• coronary arteries are absent from the compact vemtricular myocardium, specifically the deep myocardium; coronary veins occupy a larger area of the subepicardium

hemorrhage ( J:178290 )

• at E13.5 most embryos display body hemorrhages and die shortly afterwards

hemopericardium ( J:178290 )

• at E13.5 most embryos display pericardial hemorrhages and die shortly afterwards

homeostasis/metabolism

hemopericardium ( J:178290 )

• at E13.5 most embryos display pericardial hemorrhages and die shortly afterwards

muscle

thin myocardium compact layer ( J:178290 )

• compact myocardium thickness is signficantly reduced in mutants

• number of nuclei is lower (25-30%) at E13.5, with reduction in proliferating cells observed compared to control

myocardium hypoplasia ( J:178290 )

• reduction in number of smooth muscle cells in the compact myocardium is observed at E13.5

Genotype
MGI:5316087

cn2

• Allelic Composition: Gt(ROSA)26Sor^{tm1(Notch1)Dam}/Gt(ROSA)26Sor⁺; Tg(Wt1-cre)#Jbeb/0
• Genetic Background: involves: 129S4/SvJaeSor

mortality/aging

lethality throughout fetal growth and development ( J:178290 )

• mice die at E15.5

cardiovascular system

thin myocardium compact layer ( J:178290 )

• reduced subepicardium thickness is detected at E14.5

hemopericardium ( J:178290 )

• death is associated with pericardial bleeding (suggesting cardiac failure as cause of death)

abnormal epicardium morphology ( J:178290 )

• gaps in epicardium are usually associated with cysts and epicardial blistering

homeostasis/metabolism

hemopericardium ( J:178290 )

• death is associated with pericardial bleeding (suggesting cardiac failure as cause of death)

muscle

thin myocardium compact layer ( J:178290 )

• reduced subepicardium thickness is detected at E14.5