Phenotypes associated with this allele

• Allele Symbol: Sstr5^tm1Fcb
• Allele Name: targeted mutation 1, F Charles Brunicardi
• Allele ID: MGI:5303078
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Sstr5^tm1Fcb/Sstr5^tm1Fcb	involves: 129	MGI:5303085
hm2	Sstr5^tm1Fcb/Sstr5^tm1Fcb	involves: 129 * C57BL/6J	MGI:3051946
cn3	Sstr5^tm1Fcb/Sstr5^tm2Fcb Tg(Ins2-cre)1Heed/0	involves: 129S7/SvEvBrd * C57BL/6 * CBA	MGI:3603657
cx4	Abcc8^tm1Jbry/Abcc8^tm1Jbry Sstr5^tm1Fcb/Sstr5^tm1Fcb	involves: 129X1/SvJ	MGI:5303086

Genotype
MGI:5303085

hm1

• Allelic Composition: Sstr5^tm1Fcb/Sstr5^tm1Fcb
• Genetic Background: involves: 129

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

abnormal pancreatic alpha cell morphology ( J:179577 )

• increased central glucagon staining

• insulin and somatostatin staining normal

increased insulin secretion ( J:179577 )

• elevated insulin secretion

• insulin levels elevated over wild type controls 30 minutes after glucose injection but back to normal at 60 minutes

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:179577 )

N • basal glucose levels are like wild type controls

increased insulin secretion ( J:179577 )

• elevated insulin secretion

• insulin levels elevated over wild type controls 30 minutes after glucose injection but back to normal at 60 minutes

Genotype
MGI:3051946

hm2

• Allelic Composition: Sstr5^tm1Fcb/Sstr5^tm1Fcb
• Genetic Background: involves: 129 * C57BL/6J

endocrine/exocrine glands

decreased pancreatic beta cell number ( J:91762 )

• a significant decrease in the number of insulin positive cells is seen

Genotype
MGI:3603657

cn3

• Allelic Composition: Sstr5^tm1Fcb/Sstr5^tm2Fcb; Tg(Ins2-cre)1Heed/0
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6 * CBA

endocrine/exocrine glands

decreased pancreatic delta cell number ( J:99786 )

• the number of somatostatin+ delta cells is decreased to 7% and 3% of the islets cells in female and male homozygotes, respectively, compared to 13% and 9% in female and male wild-type mice

decreased insulin secretion ( J:99786 )

• when islets are incubated in vitro in a high glucose solution levels of glucose-stimulated insulin secretion are significantly decreased

• at 3 months of age 15 minutes after an injection of glucose insulin levels are not increased unlike in control mice

increased insulin secretion ( J:99786 )

• serum C-peptide levels are increased at 3 and 12 months of age suggesting an increase in beta cell insulin release

homeostasis/metabolism

decreased insulin secretion ( J:99786 )

• when islets are incubated in vitro in a high glucose solution levels of glucose-stimulated insulin secretion are significantly decreased

• at 3 months of age 15 minutes after an injection of glucose insulin levels are not increased unlike in control mice

increased insulin secretion ( J:99786 )

• serum C-peptide levels are increased at 3 and 12 months of age suggesting an increase in beta cell insulin release

decreased circulating glucose level ( J:99786 )

• at 12 months but not at 3 months of age, fasting blood glucose levels are decreased

increased circulating insulin level ( J:99786 )

• at 12 months but not at 3 months of age, basal insulin levels are increased

impaired glucose tolerance ( J:99786 )

• at 12 months but not at 3 months of age, the increase in serum glucose levels is much greater and the increase in serum insulin levels is decreased compared to wild-type mice

abnormal insulin clearance ( J:99786 )

• at 3 months of age after insulin injection blood glucose and insulin levels return to normal levels sooner than in control mice

Genotype
MGI:5303086

cx4

• Allelic Composition: Abcc8^tm1Jbry/Abcc8^tm1Jbry; Sstr5^tm1Fcb/Sstr5^tm1Fcb
• Genetic Background: involves: 129X1/SvJ

endocrine/exocrine glands

abnormal insulin secretion ( J:179577 )

• insulin levels fail to rise 30 minutes after glucose injection as they do in wild type controls

decreased insulin secretion ( J:179577 )

• insulin levels near zero

homeostasis/metabolism

abnormal insulin secretion ( J:179577 )

• insulin levels fail to rise 30 minutes after glucose injection as they do in wild type controls

decreased insulin secretion ( J:179577 )

• insulin levels near zero

decreased circulating glucose level ( J:179577 )

• significantly lower glucose levels after 16 hours of fasting compared to wild type controls

• basal levels are like wild type controls

increased circulating glucose level ( J:179577 )

• very significantly elevated glucose at both 30 and 60 minutes after glucose injection