Phenotypes associated with this allele

• Allele Symbol: Cstf2^{Gt(IST10905E6)Tigm}
• Allele Name: gene trap IST10905E6, Texas A&M Institute for Genomic Medicine
• Allele ID: MGI:5145849
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
ot1	Cstf2^Gt(IST10905E6)Tigm/Y	C57BL/6N-Cstf2^Gt(IST10905E6)Tigm	MGI:5780581

Genotype
MGI:5780581

ot1

• Allelic Composition: Cstf2^{Gt(IST10905E6)Tigm}/Y
• Genetic Background: C57BL/6N-Cstf2^{Gt(IST10905E6)Tigm}

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

embryo

abnormal endoderm development ( J:223072 )

• mutant ESCs differentiated into embryoid bodies (EBs) show a significant reduction in the expression of both primitive and definitive endoderm markers, suggesting disruption of endoderm differentiation

• in addition to a decrease in spontaneous beating and expression of cardiac markers, mutant ESC-derived cardiomyocytes show significantly reduced primitive and definitive endodermal markers relative to wild-type cells, although mesoderm markers are increased

abnormal mesoderm development ( J:223072 )

• mutant ESCs differentiated into EBs exhibit a significant increase in the expression of mesodermal markers

abnormal pluripotent precursor cell morphology ( J:223072 )

• despite increased expression of mesodermal markers, mutant ESCs show a severe defect in cardiomyocyte differentiation, with only 2-5% EBs showing rhythmic beating along with a significant decrease in the expression of cardiac and endoderm markers relative to wild-type cells

• spontaneous beating and expression of cardiac and endoderm markers can be rescued by addition of conditioned medium from extraembryonic endodermal (XEN) stem cells, indicating that mesodermal and post-mesodermal potential of these cells is normal

• mutant ESCs show normal in vitro differentiation into neuronal progenitors (an ectodermal lineage)

absent blastocoele ( J:223072 )

• in vitro, mutant ESCs differentiated into embryoid bodies (EBs) fail to cavitate, unlike wild-type EBs