Phenotypes associated with this allele

• Allele Symbol: Tmem108^tm1Lex
• Allele Name: targeted mutation 1, Lexicon Pharmaceuticals
• Allele ID: MGI:5007368
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tmem108^tm1Lex/Tmem108^tm1Lex	involves: 129S5/SvEvBrd	MGI:5881948

Genotype
MGI:5881948

hm1

• Allelic Composition: Tmem108^tm1Lex/Tmem108^tm1Lex
• Genetic Background: involves: 129S5/SvEvBrd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

impaired contextual conditioning behavior ( J:239156 )

• while mutants show similar freezing response to footshocks during training, they show less freezing time in the absence of footshocks when reintroduced to the same cage 24 hours later, suggesting suppressed fear memory consolidation

abnormal spatial working memory ( J:239156 )

• mice show decreased numbers of spontaneous alternations in the Y maze, indicating impaired spatial recognition memory

nervous system

abnormal hippocampus granule cell morphology ( J:239156 )

• dentate gyrus granule neurons show decreased AMPA receptor surface levels

abnormal dendritic spine morphology ( J:239156 )

• dendritic spine density in the molecular layer is reduced

• decrease in spine width and increase in spine length of dentate gyrus granule neuron dendrites

abnormal dentate gyrus neuron dendrite morphology ( J:239156 )

• decrease in spine width and increase in spine length of dentate gyrus granule neuron dendrites

• however, hippocampal organization, number of granule neurons in the dentate gyrus, and dendrite length and complexity of dentate gyrus granule cells are normal

reduced sensorimotor gating ( J:239156 )

• prepulse inhibition is substantially lower in mutants indicating impaired sensorimotor gating

abnormal CNS synaptic transmission ( J:239156 )

• dentate gyrus granule neurons show impaired excitatory synaptic activity and compromised glutamatergic transmission

abnormal excitatory postsynaptic currents ( J:239156 )

• postsynaptic current (sEPSC) charge transfer, but normal spontaneous inhibitory postsynaptic current (sIPSC) charge transfer, resulting in an almost 50% decrease in sEPSC/sIPSC ratio

decreased excitatory postsynaptic current amplitude ( J:239156 )

• amplitudes of evoked EPSCs, but not evoked IPSCs, are reduced when the medial performant pathway is stimulated at gradual increasing intensity

• however, paired-pulse ratios of evoked EPSC in dentate gyrus granule neurons are similar to wild-type neurons, indicating little effect on glutamate release

abnormal miniature excitatory postsynaptic currents ( J:239156 )

• the frequency and amplitude of miniature EPSCs in dentate gyrus neurons are decreased

• however, miniature IPSCs are normal

decreased prepulse inhibition ( J:239156 )

• prepulse inhibition is substantially lower in mutants

• however, baseline startle responses are normal, indicating normal hearing and acoustic startle reflex