Phenotypes associated with this allele

• Allele Symbol: Col1a1^{tm11(tetO*-RFP/RNAi:Rpa3)Slowe}
• Allele Name: targeted mutation 11, Scott Lowe
• Allele ID: MGI:4999906
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Col1a1^tm11(tetO*-RFP/RNAi:Rpa3)Slowe/Col1a1^+ Gt(ROSA)26Sor^tm1(rtTA*M2)Jae/Gt(ROSA)26Sor^+	involves: 129S4/SvJae * C57BL/6	MGI:5000012
cx2	Col1a1^tm11(tetO*-RFP/RNAi:Rpa3)Slowe/Col1a1^+ Gt(ROSA)26Sor^tm1(rtTA*M2)Jae/Gt(ROSA)26Sor^tm1(rtTA*M2)Jae	involves: 129S4/SvJae * C57BL/6	MGI:5000013
cx3	Col1a1^tm11(tetO*-RFP/RNAi:Rpa3)Slowe/Col1a1^+ Gt(ROSA)26Sor^tm1(rtTA*M2)Jae/Gt(ROSA)26Sor^+ Tg(CMV-rtTA)4Bjd/0	involves: 129S4/SvJae * C57BL/6 * NMRI	MGI:5000014

Genotype
MGI:5000012

cx1

• Allelic Composition: Col1a1^{tm11(tetO*-RFP/RNAi:Rpa3)Slowe}/Col1a1⁺; Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:171348 )

• when treated in utero with doxycycline, mice die shortly after birth

cellular

abnormal cell cycle ( J:171348 )

• doxycycline-treated mouse embryonic fibroblasts arrest in S phase with increased DNA content compared with untreated cells

early cellular replicative senescence ( J:171348 )

• doxycycline-treated mouse embryonic fibroblasts exhibit early cellular replicative senescence compared with untreated cells

• however, withdrawal of doxcycline restores proliferation

normal phenotype

no abnormal phenotype detected ( J:171348 )

• mice treated with doxycycline during adulthood exhibit no abnormal phenotype

Genotype
MGI:5000013

cx2

• Allelic Composition: Col1a1^{tm11(tetO*-RFP/RNAi:Rpa3)Slowe}/Col1a1⁺; Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}/Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}
• Genetic Background: involves: 129S4/SvJae * C57BL/6

mortality/aging

premature death ( J:171348 )

• all mice treated with doxycycline between 5 and 10 weeks of age die prematurely

digestive/alimentary system

abnormal small intestinal crypt cell physiology ( J:171348 )

• doxycycline-treated adult mice exhibit atrophy of intestinal epithelium unlike control mice

• however, withdrawal of doxycycline reverses villus atrophy

abnormal small intestinal crypt cell proliferation ( J:171348 )

• doxycycline-treated adult mice exhibit decreased proliferation and cell cycle arrest in the intestinal epithelium unlike control mice

hematopoietic system

decreased erythroid progenitor cell number ( J:171348 )

• doxycycline-treated adult mice exhibit decreased erythroid precursors in the bone marrow unlike in control mice

growth/size/body

weight loss ( J:171348 )

• in doxycycline-treated adult mice

• however, mice regain weight after withdrawal of doxycycline

cellular

abnormal small intestinal crypt cell proliferation ( J:171348 )

• doxycycline-treated adult mice exhibit decreased proliferation and cell cycle arrest in the intestinal epithelium unlike control mice

Genotype
MGI:5000014

cx3

• Allelic Composition: Col1a1^{tm11(tetO*-RFP/RNAi:Rpa3)Slowe}/Col1a1⁺; Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}/Gt(ROSA)26Sor⁺; Tg(CMV-rtTA)4Bjd/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * NMRI

mortality/aging

premature death ( J:171348 )

• most mice treated with doxycycline between 5 and 10 weeks of age die prematurely

growth/size/body

weight loss ( J:171348 )

• in doxycycline-treated mice