Phenotypes associated with this allele

• Allele Symbol: Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng
• Allele Name: transgene insertion 6, Guoping Feng
• Allele ID: MGI:4950478
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Chat/Slc18a3^tm1.1Vpra/Chat/Slc18a3^tm1.1Vpra Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng/0	involves: 129S4/SvJae * C57BL/6 * CBA	MGI:5491874
cx2	Chat/Slc18a3^tm1.1Vpra/Chat/Slc18a3^tm1.1Vpra Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng/0	involves: 129S4/SvJae * C57BL/6 * CBA	MGI:5491876
tg3	Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng/0	B6.Cg-Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng/J	MGI:5491602
tg4	Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng/0	involves: 129S4/SvJae * C57BL/6 * CBA * FVB/N	MGI:5585596

Genotype
MGI:5491874

cx1

• Allelic Composition: Chat/Slc18a3^tm1.1Vpra/Chat/Slc18a3^tm1.1Vpra; Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

mortality/aging ( J:197166 )

N • mice are viable and survive to adulthood in contrast to mice without the transgene

Genotype
MGI:5491876

cx2

• Allelic Composition: Chat/Slc18a3^tm1.1Vpra/Chat/Slc18a3^tm1.1Vpra; Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * CBA

mortality/aging

mortality/aging ( J:197166 )

N • mice are viable and survive to adulthood in contrast to mice without the transgene

Genotype
MGI:5491602

tg3

• Allelic Composition: Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng/0
• Genetic Background: B6.Cg-Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng/J

behavior/neurological

impaired cued conditioning behavior ( J:197166 )

• mice did not improve performance in the cued version of Morris water maze

abnormal motor learning ( J:197166 )

• mice do not improve time spent or distance travelled on rotarod as compared to controls

abnormal spatial learning ( J:197166 )

• mice visit target hole less than controls in Barnes maze test

• mice show no preference for target quadrant in Morris water maze during the probe trial, although they perform similar to controls in acquisition phase

abnormal spatial working memory ( J:197166 )

• mice revisit arms of Y-maze more often than controls, with less spontaneous alternations

increased fluid intake ( J:197166 )

• mice consume more food and water during the dark cycle than controls

increased food intake ( J:197166 )

• mice consume more food and water during the dark cycle than controls

homeostasis/metabolism

enhanced exercise endurance ( J:197166 )

• mice run almost twice as much on treadmill as controls

Genotype
MGI:5585596

tg4

• Allelic Composition: Tg(Chat-COP4*H134R/EYFP,Slc18a3)6Gfng/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * CBA * FVB/N

behavior/neurological

enhanced behavioral response to addictive substance ( J:213335 )

induced hyperactivity ( J:213335 )

• following a first injection of low dose D-amphetamine mice spent less time in slow versus fast locomotion and exhibited an increase in distance travelled as compared to wild-type

increased stereotypic behavior ( J:213335 )

• 20 minutes after injection with high dose D-amphetamine transgenic mice exhibit severe and confined stereotypic behaviors such as floor/wall sniffing

nervous system

abnormal brain interneuron morphology ( J:213335 )

• increased number of cholinergic interneurons, however, compartmental distribution of processes is similar to control

abnormal striatum morphology ( J:213335 )

• cholinergic neuropil of the striatum is denser than neuropil found in controls