Phenotypes associated with this allele

• Allele Symbol: Rhoa^tm1Jrel
• Allele Name: targeted mutation 1, Joao B Relvas
• Allele ID: MGI:4950374
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Rhoa^tm1Jrel/Rhoa^tm1Jrel Rhpn1^tm1Ktry/Rhpn1^tm1Ktry Tg(NPHS2-cre)295Lbh/0	involves: 129X1/SvJ * C57BL/6 * SJL	MGI:6360618
cn2	Rhoa^tm1Jrel/Rhoa^tm1Jrel Tg(Pou3f4-cre)32Cren/0	involves: CD-1	MGI:4950375

Genotype
MGI:6360618

cn1

• Allelic Composition: Rhoa^tm1Jrel/Rhoa^tm1Jrel; Rhpn1^tm1Ktry/Rhpn1^tm1Ktry; Tg(NPHS2-cre)295Lbh/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

decreased body weight ( J:276466 )

• reduced body weight by 3 months of age

homeostasis/metabolism

increased circulating creatinine level ( J:276466 )

renal/urinary system

abnormal kidney morphology ( J:276466 )

• mice exhibit a more severe kidney injury than single Rhpn1 homozygotes

podocyte foot process effacement ( J:276466 )

• complete podocyte effacement

glomerulosclerosis ( J:276466 )

• segmental and global focal segmental glomerulosclerosis

renal interstitial fibrosis ( J:276466 )

dilated renal tubule ( J:276466 )

renal cast ( J:276466 )

• protein casts

Genotype
MGI:4950375

cn2

• Allelic Composition: Rhoa^tm1Jrel/Rhoa^tm1Jrel; Tg(Pou3f4-cre)32Cren/0
• Genetic Background: involves: CD-1

mortality/aging

embryonic lethality, complete penetrance ( J:171199 )

• mice die at late embryonic stages

nervous system

nervous system phenotype ( J:171199 )

N • mice exhibit normal basolateral and radial fibers, and glia- and motor neuron generation

increased spinal cord apoptosis ( J:171199 )

• increased apoptosis in the spinal cord at E11.5 and E12.5

premature neuronal precursor differentiation ( J:171199 )

• at E13.5, cultured neurospheres exhibit reduced self-renewal compared with wild-type cells

abnormal neuronal precursor proliferation ( J:171199 )

• at E10.5 and E11.5, spinal cord cells exhibit early cell-cycle exit compared with wild-type cells

abnormal neural tube ventricular layer morphology ( J:171199 )

• at E11.5, spinal cords lack a well-organized ventricular zone and exhibit dysplasia, likely due to neuroepithelial cells invading the lumen of the neural tube, compared with wild-type mice

• rossette-like structures are present in the ventricular zone of the spinal cord unlike in wild-type mice

• at E14.5, the ventricular zone and neural tube lumen are missing unlike in wild-type mice

embryo

abnormal neural tube ventricular layer morphology ( J:171199 )

• at E11.5, spinal cords lack a well-organized ventricular zone and exhibit dysplasia, likely due to neuroepithelial cells invading the lumen of the neural tube, compared with wild-type mice

• rossette-like structures are present in the ventricular zone of the spinal cord unlike in wild-type mice

• at E14.5, the ventricular zone and neural tube lumen are missing unlike in wild-type mice

cellular

increased spinal cord apoptosis ( J:171199 )

• increased apoptosis in the spinal cord at E11.5 and E12.5

premature neuronal precursor differentiation ( J:171199 )

• at E13.5, cultured neurospheres exhibit reduced self-renewal compared with wild-type cells

abnormal neuronal precursor proliferation ( J:171199 )

• at E10.5 and E11.5, spinal cord cells exhibit early cell-cycle exit compared with wild-type cells