Phenotypes associated with this allele

• Allele Symbol: Spic^tm1Kmm
• Allele Name: targeted mutation 1, Kenneth M Murphy
• Allele ID: MGI:4939280
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Spic^tm1Kmm/Spic^tm1Kmm	involves: 129S6/SvEvTac * C57BL/6	MGI:4939361

Genotype
MGI:4939361

hm1

• Allelic Composition: Spic^tm1Kmm/Spic^tm1Kmm
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

prenatal lethality, incomplete penetrance ( J:143900 )

• only 9% of pups born to heterozygote parents are homozyogtes instead of the expected 25%

immune system

impaired macrophage phagocytosis ( J:143900 )

• clearance of senescent red blood cells in the spleen is impaired

increased spleen weight ( J:143900 )

• by 16 weeks of age, spleen weight is about 50% more than in controls

• this increase in weight is attributable to an increase in stores of iron in the spleen

abnormal macrophage morphology ( J:143900 )

• spleen red pulp macrophages (RPM), characterized by F4/80^hiCD68⁺CD11b^lo expression with strong autofluorescence in flow cytometry, are selectively lost in these mice

• bone marrow chimera experiments demonstrate the defect in RPM generation is intrinsic to haematopoietic cells

• other macrophage population numbers, including marginal zone and metallophillic macrophages, are normal in these mice

increased spleen iron level ( J:143900 )

• iron levels are about double in the spleen compared to controls

homeostasis/metabolism

increased spleen iron level ( J:143900 )

• iron levels are about double in the spleen compared to controls

hematopoietic system

impaired macrophage phagocytosis ( J:143900 )

• clearance of senescent red blood cells in the spleen is impaired

increased spleen weight ( J:143900 )

• by 16 weeks of age, spleen weight is about 50% more than in controls

• this increase in weight is attributable to an increase in stores of iron in the spleen

abnormal macrophage morphology ( J:143900 )

• spleen red pulp macrophages (RPM), characterized by F4/80^hiCD68⁺CD11b^lo expression with strong autofluorescence in flow cytometry, are selectively lost in these mice

• bone marrow chimera experiments demonstrate the defect in RPM generation is intrinsic to haematopoietic cells

• other macrophage population numbers, including marginal zone and metallophillic macrophages, are normal in these mice

increased spleen iron level ( J:143900 )

• iron levels are about double in the spleen compared to controls

growth/size/body

increased spleen weight ( J:143900 )

• by 16 weeks of age, spleen weight is about 50% more than in controls

• this increase in weight is attributable to an increase in stores of iron in the spleen

cellular

impaired macrophage phagocytosis ( J:143900 )

• clearance of senescent red blood cells in the spleen is impaired