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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Lpar4tm1Sati
targeted mutation 1, Satoshi Ishii
MGI:4868215
Summary 7 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Lpar4tm1Sati/Lpar4tm1Sati involves: C57BL/6 MGI:4868233
ht2
Lpar4tm1Sati/Lpar4+ involves: C57BL/6 MGI:7865352
cx3
Lpar4tm1Sati/Y
Lpar6tm1Dgen/Lpar6+
involves: 129P2/OlaHsd * C57BL/6 MGI:7861282
cx4
Lpar4tm1Sati/Y
Lpar6tm1Dgen/Lpar6tm1Dgen
involves: 129P2/OlaHsd * C57BL/6 MGI:7861279
cx5
Lpar4tm1Sati/Lpar4tm1Sati
Lpar6tm1Dgen/Lpar6tm1Dgen
involves: 129P2/OlaHsd * C57BL/6 MGI:7861277
cx6
Lpar4tm1Sati/Lpar4+
Lpar6tm1Dgen/Lpar6tm1Dgen
involves: 129P2/OlaHsd * C57BL/6 MGI:7861281
ot7
Lpar4tm1Sati/Y involves: C57BL/6 MGI:4868231


Genotype
MGI:4868233
hm1
Allelic
Composition
Lpar4tm1Sati/Lpar4tm1Sati
Genetic
Background
involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lpar4tm1Sati mutation (0 available); any Lpar4 mutation (4 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• fewer than expected mice are present at weaning
• fewer than expected mice are present between E12.5 and E18.5

cardiovascular system
N
• i.v. injected FITC-dextran is mostly retained within the vasculature at levels similar to those in wild-type controls (J:253134)
• mice exhibit normal mean arterial blood pressure (MAP) and heart rate under normal conditions (J:275252)
• mice with bleeding exhibit dilated ventral skin blood vessels with poor mural cell coverage compared with wild-type mice
• mice with bleeding exhibit dilated ventral skin blood vessels with poor mural cell coverage compared with wild-type mice
• mice with bleeding exhibit dilated ventral skin blood vessels with poor mural cell coverage compared with wild-type mice
• in some mice
• at E14.5, some mice exhibit subcutaneous hemorrhage unlike wild-type mice
• at E18.5, some mice exhibit hemorrhage in the neck, head, limbs, and other areas of the body unlike wild-type mice
• at E18.5, some mice exhibit hemorrhagic lesions in pericardial cavities, periocular tissues, salivary glands, and subcutaneous tissues unlike wild-type mice
• however, blood coagulation is normal
• at E18.5, 4 of 17 mice exhibit thymic hemorrhage unlike wild-type mice
• in 14 of 17 mice at E18.5
• in 1 of 17 mice at E18.5
• after i.v. injection of lysophosphatidic acid (18:1-LPA), mice show attenuated LPA-induced transient hypertension with a slightly but significantly lower response to various LPA dosages than in similarly treated wild-type controls
• however, mice exhibit normal hypertensive responses to phenylephrine and norepinephrine

homeostasis/metabolism
N
• high endothelial venules (HEVs) and fibroblastic reticular cells show normal expression of ENPP2/ATX (ectonucleotide pyrophosphatase/phosphodiesterase 2, also known as autotaxin), indicating normal local production of lysophosphatidic acid (LPA)
• some mice exhibit effusions in the body surface unlike wild-type mice
• in some mice
• in 14 of 17 mice at E18.5
• in some mice at E14.5 and E18.5

growth/size/body
• some mice at E10.5 and E12.5
• in some mice at E18.5
• some mice at E14.5

embryo
• at E12.5, one third of mice exhibit various developmental anomalies unlike wild-type mice
• some mice at E10.5 and E12.5

skeleton
• in some mice at E18.5

craniofacial
• in some mice at E18.5

integument
• mice with bleeding exhibit dilated ventral skin blood vessels with poor mural cell coverage compared with wild-type mice
• in some mice at E14.5 and E18.5

muscle
• mice with bleeding exhibit dilated ventral skin blood vessels with poor mural cell coverage compared with wild-type mice

respiratory system
• in 1 of 17 mice at E18.5

immune system
N
• mice show normal formation of high endothelial venules (HEVs), B-cell follicles, and T-cell areas in the lymph nodes (LNs), with no abnormalities in CD4, CD8 or B220 expression
• overall tissue architecture and blood vessel distribution pattern is normal in peripheral LNs
• mice show a significantly greater lymphocyte accumulation within the HEV endothelial cell (EC) layer of the inguinal LN (ILN) than Lpar4tm1Sati heterozygotes and wild-type controls
• increased lymphocyte accumulation is also noted within the EC layer of PNAd+ HEV ECs in the mesenteric LN (MLN), but to a lesser extent than in the PNAd+ HEV ECs of peripheral LNs
• TEM analysis of HEVs in the ILNs shows more lymphocytes in the HEV EC layer including the perivenular channels, occasionally causing narrowing of the luminal surface of HEV ECs
• most accumulated lymphocytes are nested between the ECs and the underlying basal lamina
• most pockets in the HEV EC layer (areas where lymphocytes reside for several minutes before they enter the LN parenchyma) contain more lymphocytes (occasionally >10 in a single pocket), whereas wild-type pockets typically house up to 4-5 lymphocytes
• after adoptive transfer, the number of migrated wild-type GFP+ donor cells is mildly reduced in the ILN, but not in spleen, with T-cell migration more strongly affected than B-cell migration in the ILN
• in a trafficking assay using whole-mount analysis of LNs, the density of transferred lymphocytes around HEVs (= number of donor cells within the HEV EC layer / number of recently extravasated cells) as well as the HEV surface area and volume are significantly increased, indicating delayed lymphocyte transmigration across the HEV wall
• lymph sacs and lymphatic vessels are dilated compared to in wild-type mice
• however, lymphatic endothelial cell proliferation is normal

hematopoietic system
• mice show a significantly greater lymphocyte accumulation within the HEV endothelial cell (EC) layer of the inguinal LN (ILN) than Lpar4tm1Sati heterozygotes and wild-type controls
• increased lymphocyte accumulation is also noted within the EC layer of PNAd+ HEV ECs in the mesenteric LN (MLN), but to a lesser extent than in the PNAd+ HEV ECs of peripheral LNs
• TEM analysis of HEVs in the ILNs shows more lymphocytes in the HEV EC layer including the perivenular channels, occasionally causing narrowing of the luminal surface of HEV ECs
• most accumulated lymphocytes are nested between the ECs and the underlying basal lamina
• most pockets in the HEV EC layer (areas where lymphocytes reside for several minutes before they enter the LN parenchyma) contain more lymphocytes (occasionally >10 in a single pocket), whereas wild-type pockets typically house up to 4-5 lymphocytes
• after adoptive transfer, the number of migrated wild-type GFP+ donor cells is mildly reduced in the ILN, but not in spleen, with T-cell migration more strongly affected than B-cell migration in the ILN
• in a trafficking assay using whole-mount analysis of LNs, the density of transferred lymphocytes around HEVs (= number of donor cells within the HEV EC layer / number of recently extravasated cells) as well as the HEV surface area and volume are significantly increased, indicating delayed lymphocyte transmigration across the HEV wall

cellular
• mice show a significantly greater lymphocyte accumulation within the HEV endothelial cell (EC) layer of the inguinal LN (ILN) than Lpar4tm1Sati heterozygotes and wild-type controls
• increased lymphocyte accumulation is also noted within the EC layer of PNAd+ HEV ECs in the mesenteric LN (MLN), but to a lesser extent than in the PNAd+ HEV ECs of peripheral LNs
• TEM analysis of HEVs in the ILNs shows more lymphocytes in the HEV EC layer including the perivenular channels, occasionally causing narrowing of the luminal surface of HEV ECs
• most accumulated lymphocytes are nested between the ECs and the underlying basal lamina
• most pockets in the HEV EC layer (areas where lymphocytes reside for several minutes before they enter the LN parenchyma) contain more lymphocytes (occasionally >10 in a single pocket), whereas wild-type pockets typically house up to 4-5 lymphocytes
• after adoptive transfer, the number of migrated wild-type GFP+ donor cells is mildly reduced in the ILN, but not in spleen, with T-cell migration more strongly affected than B-cell migration in the ILN
• in a trafficking assay using whole-mount analysis of LNs, the density of transferred lymphocytes around HEVs (= number of donor cells within the HEV EC layer / number of recently extravasated cells) as well as the HEV surface area and volume are significantly increased, indicating delayed lymphocyte transmigration across the HEV wall




Genotype
MGI:7865352
ht2
Allelic
Composition
Lpar4tm1Sati/Lpar4+
Genetic
Background
involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lpar4tm1Sati mutation (0 available); any Lpar4 mutation (4 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• lymphocyte accumulation within the HEV endothelial cell (EC) layer of the inguinal LNs (ILNs) is significantly greater than in wild-type controls but significantly less than in Lpar4tm1Sati homozygotes

hematopoietic system
• lymphocyte accumulation within the HEV endothelial cell (EC) layer of the inguinal LNs (ILNs) is significantly greater than in wild-type controls but significantly less than in Lpar4tm1Sati homozygotes

cellular
• lymphocyte accumulation within the HEV endothelial cell (EC) layer of the inguinal LNs (ILNs) is significantly greater than in wild-type controls but significantly less than in Lpar4tm1Sati homozygotes




Genotype
MGI:7861282
cx3
Allelic
Composition
Lpar4tm1Sati/Y
Lpar6tm1Dgen/Lpar6+
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lpar4tm1Sati mutation (0 available); any Lpar4 mutation (4 available)
Lpar6tm1Dgen mutation (0 available); any Lpar6 mutation (28 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• fewer than expected male mice are born

reproductive system
N
• male mice are fertile




Genotype
MGI:7861279
cx4
Allelic
Composition
Lpar4tm1Sati/Y
Lpar6tm1Dgen/Lpar6tm1Dgen
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lpar4tm1Sati mutation (0 available); any Lpar4 mutation (4 available)
Lpar6tm1Dgen mutation (0 available); any Lpar6 mutation (28 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• no double knockout male mice are born




Genotype
MGI:7861277
cx5
Allelic
Composition
Lpar4tm1Sati/Lpar4tm1Sati
Lpar6tm1Dgen/Lpar6tm1Dgen
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lpar4tm1Sati mutation (0 available); any Lpar4 mutation (4 available)
Lpar6tm1Dgen mutation (0 available); any Lpar6 mutation (28 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• no double knockout female mice are born




Genotype
MGI:7861281
cx6
Allelic
Composition
Lpar4tm1Sati/Lpar4+
Lpar6tm1Dgen/Lpar6tm1Dgen
Genetic
Background
involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lpar4tm1Sati mutation (0 available); any Lpar4 mutation (4 available)
Lpar6tm1Dgen mutation (0 available); any Lpar6 mutation (28 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• fewer than expected female mice are born

reproductive system
N
• female mice are fertile




Genotype
MGI:4868231
ot7
Allelic
Composition
Lpar4tm1Sati/Y
Genetic
Background
involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lpar4tm1Sati mutation (0 available); any Lpar4 mutation (4 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• fewer than expected mice are present at weaning
• fewer than expected mice are present between E12.5 and E18.5

cardiovascular system
N
• i.v. injected FITC-dextran is mostly retained within the vasculature at levels similar to those in wild-type controls
• mice with bleeding exhibit dilated ventral skin blood vessels with poor mural cell coverage compared with wild-type mice
• mice with bleeding exhibit dilated ventral skin blood vessels with poor mural cell coverage compared with wild-type mice
• mice with bleeding exhibit dilated ventral skin blood vessels with poor mural cell coverage compared with wild-type mice
• in some mice
• at E14.5, some mice exhibit subcutaneous hemorrhage unlike wild-type mice
• at E18.5, some mice exhibit hemorrhage in the neck, head, limbs, and other areas of the body unlike wild-type mice
• at E18.5, some mice exhibit hemorrhagic lesions in pericardial cavities, periocular tissues, salivary glands, and subcutaneous tissues unlike wild-type mice
• however, blood coagulation is normal
• at E18.5, 4 of 17 mice exhibit thymic hemorrhage unlike wild-type mice
• in 14 of 17 mice at E18.5
• in 1 of 17 mice at E18.5

homeostasis/metabolism
N
• high endothelial venules (HEVs) and fibroblastic reticular cells show normal expression of ENPP2/ATX (ectonucleotide pyrophosphatase/phosphodiesterase 2, also known as autotaxin), indicating normal local production of lysophosphatidic acid (LPA)
• some mice exhibit effusions in the body surface unlike wild-type mice
• in some mice
• in 14 of 17 mice at E18.5
• in some mice at E14.5 and E18.5

growth/size/body
N
• 8-week-old males exhibit normal total body weight relative to wild-type controls
• some mice at E10.5 and E12.5
• in some mice at E18.5
• some mice at E14.5

embryo
• at E12.5, one third of mice exhibit various developmental anomalies unlike wild-type mice
• some mice at E10.5 and E12.5

skeleton
• in some mice at E18.5

craniofacial
• in some mice at E18.5

immune system
N
• mice show normal formation of high endothelial venules (HEVs), B-cell follicles, and T-cell areas in the lymph nodes (LNs), with no abnormalities in CD4, CD8 or B220 expression in LN cells
• 8-week-old mice show no significant differences in the total cell number in spleen, mesenteric LNs (MLNs), inguinal LNs (ILNs) and popliteal LNs (PLNs) relative to wild-type controls
• overall tissue architecture and blood vessel distribution pattern is normal in peripheral LNs
• mice show a significantly greater lymphocyte accumulation within the HEV endothelial cell (EC) layer of the inguinal LN (ILN) than wild-type controls
• increased lymphocyte accumulation is also noted within the EC layer of PNAd+ HEV ECs in the mesenteric LN (MLN), but to a lesser extent than in the PNAd+ HEV ECs of peripheral LNs
• lymph sacs and lymphatic vessels are dilated compared to in wild-type mice
• however, lymphatic endothelial cell proliferation is normal

integument
• mice with bleeding exhibit dilated ventral skin blood vessels with poor mural cell coverage compared with wild-type mice
• in some mice at E14.5 and E18.5

muscle
• mice with bleeding exhibit dilated ventral skin blood vessels with poor mural cell coverage compared with wild-type mice

respiratory system
• in 1 of 17 mice at E18.5

hematopoietic system
• mice show a significantly greater lymphocyte accumulation within the HEV endothelial cell (EC) layer of the inguinal LN (ILN) than wild-type controls
• increased lymphocyte accumulation is also noted within the EC layer of PNAd+ HEV ECs in the mesenteric LN (MLN), but to a lesser extent than in the PNAd+ HEV ECs of peripheral LNs

cellular
• mice show a significantly greater lymphocyte accumulation within the HEV endothelial cell (EC) layer of the inguinal LN (ILN) than wild-type controls
• increased lymphocyte accumulation is also noted within the EC layer of PNAd+ HEV ECs in the mesenteric LN (MLN), but to a lesser extent than in the PNAd+ HEV ECs of peripheral LNs





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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory