Phenotypes associated with this allele

• Allele Symbol: Kmt2b^tm2.1Afst
• Allele Name: targeted mutation 2.1, A Francis Stewart
• Allele ID: MGI:4867487
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Kmt2b^tm2.1Afst/Kmt2b^tm2.1Afst	involves: 129 * C57BL/6	MGI:4867489
ht2	Kmt2b^tm2.1Afst/Kmt2b^+	involves: 129 * C57BL/6	MGI:4867490

Genotype
MGI:4867489

hm1

• Allelic Composition: Kmt2b^tm2.1Afst/Kmt2b^tm2.1Afst
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reduced female fertility ( J:166778 )

♀ • lower cumulative number of pups from homozygous females over a 6-month period than that of controls

embryo

abnormal embryo development ( J:166778 )

• 30% reduction in Transcription Required Complex levels in 2-cell embryos

embryonic growth arrest ( J:166778 )

• lower percentage of mutant embryos reach the blastocyst stage in embryo cultures

• most embryos (75%) accumulate between 1-cell and 4-cell stages in embryo cultures

• higher percentage of fragmentation in embryo cultures

cellular

abnormal DNA methylation ( J:166778 )

• decreased expression of di- and tri-methylated H3K4 in the female pronucleus of homozygous 1-cell embryos

• decreased global tri-methylated H3K4 levels in homozygous zygotes

Genotype
MGI:4867490

ht2

• Allelic Composition: Kmt2b^tm2.1Afst/Kmt2b⁺
• Genetic Background: involves: 129 * C57BL/6

reproductive system

reduced female fertility ( J:166778 )

♀ • lower cumulative number of pups from mutant females over a 6-month period than that of controls

embryo

embryonic growth arrest ( J:166778 )

• few embryos reach the blastocyst stage in embryo cultures