Phenotypes associated with this allele

• Allele Symbol: Nusap1^tm1.1Gcm
• Allele Name: targeted mutation 1.1, Geert Carmeliet
• Allele ID: MGI:4833676
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nusap1^tm1.1Gcm/Nusap1^tm1.1Gcm	involves: 129/Sv * Swiss	MGI:4833677

Genotype
MGI:4833677

hm1

• Allelic Composition: Nusap1^tm1.1Gcm/Nusap1^tm1.1Gcm
• Genetic Background: involves: 129/Sv * Swiss

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality at implantation, complete penetrance ( J:164315 )

• homozygous embryos are present in expected numbers at E3.5

• no homozygous embryos at E10.5

• embryonic death around the time of implantation

embryo

abnormal inner cell mass apoptosis ( J:164315 )

• E3.5 embryos cultured in vitro for 3 days contain extensive apoptotic cells

decreased embryo size ( J:164315 )

• smaller than controls after E3.5

abnormal embryonic tissue morphology ( J:164315 )

disorganized embryonic tissue ( J:164315 )

• internal embryonic structure is disorganized subsequent to E3.5

abnormal inner cell mass morphology ( J:164315 )

• inner cell mass is reduced after E3.5

cellular

abnormal mitosis ( J:164315 )

• mitotic cells are mostly in prometaphase (72%)

• chromosomes remain dispersed in cytoplasm

abnormal mitotic spindle morphology ( J:164315 )

• spindle structures are distorted

increased mitotic index ( J:164315 )

• increased number of mitotic cells

abnormal inner cell mass apoptosis ( J:164315 )

• E3.5 embryos cultured in vitro for 3 days contain extensive apoptotic cells

growth/size/body

decreased embryo size ( J:164315 )

• smaller than controls after E3.5