Phenotypes associated with this allele

• Allele Symbol: Trim33^tm1.1Los
• Allele Name: targeted mutation 1.1, Regine Losson
• Allele ID: MGI:4830322
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Tg(CAG-cre)13Miya/0 Trim33^tm1.1Los/Trim33^tm1.2Los	involves: 129 * 129S2/SvPas * C57BL/6 * SJL	MGI:4830330
cn2	Trim33^tm1.1Los/Trim33^tm1.2Los Edil3^Tg(Sox2-cre)1Amc/Edil3^+	involves: 129S2/SvPas * C57BL/6 * CBA * SJL	MGI:4830326
cn3	Smad4^tm1Rdp/Smad4^tm1.1Rdp Trim33^tm1.1Los/Trim33^tm1.2Los Tg(CAG-cre)13Miya/0	involves: 129S/SvEv * 129S2/SvPas * C57BL/6 * SJL	MGI:4830328

Genotype
MGI:4830330

cn1

• Allelic Composition: Tg(CAG-cre)13Miya/0; Trim33^tm1.1Los/Trim33^tm1.2Los
• Genetic Background: involves: 129 * 129S2/SvPas * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

embryo

abnormal embryo development ( J:163858 )

• stated to be indistinguishable from mice homozygous for Trim33^tm1.2Ros

abnormal anterior visceral endoderm morphology ( J:163858 )

• expanded at E5.5

Genotype
MGI:4830326

cn2

• Allelic Composition: Trim33^tm1.1Los/Trim33^tm1.2Los; Edil3^{Tg(Sox2-cre)1Amc}/Edil3⁺
• Genetic Background: involves: 129S2/SvPas * C57BL/6 * CBA * SJL

mortality/aging

embryonic lethality, incomplete penetrance ( J:163858 )

• few embryos survive to E10

embryo

embryo phenotype ( J:163858 )

N • unlike in homozygous germline null mice, no defects are seen in the anterior visceral endoderm or the extraembryonic ectoderm

abnormal endoderm development ( J:163858 )

• expression analysis indicates an almost radial expansion of the definitive endoderm at E7.5

abnormal mesendoderm development ( J:163858 )

• duplications of anterior axial mesendoderm tissues

decreased embryo size ( J:163858 )

• seen in about 1/3 of embryos that also have a defect in primitive streak morphology

incomplete rostral neuropore closure ( J:163858 )

• at E10 the few surviving embryos display open neural folds

abnormal primitive node morphology ( J:163858 )

• expanded in surviving embryos at E8.0

• duplications of node tissues

abnormal primitive streak elongation ( J:163858 )

• about 1/3 of embryos lack an overtly elongated streak

nervous system

incomplete rostral neuropore closure ( J:163858 )

• at E10 the few surviving embryos display open neural folds

abnormal brain development ( J:163858 )

• at E10 the few surviving embryos display defective brain development

growth/size/body

decreased embryo size ( J:163858 )

• seen in about 1/3 of embryos that also have a defect in primitive streak morphology

Genotype
MGI:4830328

cn3

• Allelic Composition: Smad4^tm1Rdp/Smad4^tm1.1Rdp; Trim33^tm1.1Los/Trim33^tm1.2Los; Tg(CAG-cre)13Miya/0
• Genetic Background: involves: 129S/SvEv * 129S2/SvPas * C57BL/6 * SJL

embryo

abnormal embryo development ( J:163858 )

• identical to mice null for Smad4 alone

absent anterior visceral endoderm ( J:163858 )

• not induced at E5.5