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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Appl1tm1.2Test
targeted mutation 1.2, Joseph Testa
MGI:4454678
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Appl1tm1.2Test/Appl1tm1.2Test involves: 129X1/SvJ MGI:4838333
hm2
 		Appl1tm1.2Test/Appl1tm1.2Test involves: 129X1/SvJ * C57BL/6 MGI:4454680
cx3
 		Appl1tm1.2Test/Appl1tm1.2Test
Appl2tm1.1Test/Appl2tm1.1Test 		involves: 129X1/SvJ MGI:5766636


Genotype
MGI:4838333
hm1
Allelic
Composition		 Appl1tm1.2Test/Appl1tm1.2Test
Genetic
Background		 involves: 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Appl1tm1.2Test mutation (0 available); any Appl1 mutation (45 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
hematopoietic system phenotype ( J:227340 )
N
• at 8 weeks of age, mice exhibit normal numbers and % of lymphocytes, monocytes, neutrophils, eosinophils, and basophils relative to controls
• no pernicious anemia is observed, as WBC counts and spleen and liver size are normal
increased T cell proliferation ( J:164696 )
• slightly in response to ConA treatment
increased hematocrit ( J:227340 )
• hematocrit is slightly but significantly increased, esp. in female mice

cellular
abnormal cell physiology ( J:227340 )
• primary MEFs derived from E13.5 embryos exhibit decreased Akt activation upon stimulation with HGF
• however, in vivo, basal Akt activity is not significantly altered in stomach, liver or muscle tissues
decreased fibroblast cell migration ( J:227340 )
• primary MEFs derived from E13.5 embryos exhibit reduced HGF-induced transmembrane migration and decreased movement in a wound-healing assay as well as markedly decreased HGF-triggered invasion in a matrigel assay
• in contrast, EGF-induced migration and invasion are normal
increased T cell proliferation ( J:164696 )
• slightly in response to ConA treatment

immune system
increased T cell proliferation ( J:164696 )
• slightly in response to ConA treatment




Genotype
MGI:4454680
hm2
Allelic
Composition		 Appl1tm1.2Test/Appl1tm1.2Test
Genetic
Background		 involves: 129X1/SvJ * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Appl1tm1.2Test mutation (0 available); any Appl1 mutation (45 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
cellular phenotype ( J:159889 )
N
• mouse embryonic fibroblasts exhibit normal proliferation, survival under normal conditions, and UV-induced apoptosis
increased sensitivity to induced cell death ( J:159889 )
• mouse embryonic fibroblast cells cultured in the presence of HGF exhibit impaired survival compared with similarly treated wild-type cells
decreased fibroblast cell migration ( J:159889 )
• mouse embryonic fibroblasts exhibit reduced HGF-induced migration compared with similarly treated wild-type cells

hematopoietic system
hematopoietic system phenotype ( J:159889 )
N
• mice exhibit no obvious hematopoietic system defects




Genotype
MGI:5766636
cx3
Allelic
Composition		 Appl1tm1.2Test/Appl1tm1.2Test
Appl2tm1.1Test/Appl2tm1.1Test
Genetic
Background		 involves: 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Appl1tm1.2Test mutation (0 available); any Appl1 mutation (45 available)
Appl2tm1.1Test mutation (0 available); any Appl2 mutation (27 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
mortality/aging ( J:227340 )
N
• unexpectedly, mice are viable and show grossly normal postnatal growth and fertility, similar to single homozygotes

hematopoietic system
hematopoietic system phenotype ( J:227340 )
N
• at 8 weeks of age, mice exhibit normal numbers and % of lymphocytes, monocytes, neutrophils, eosinophils, and basophils relative to controls
• no pernicious anemia is observed, as WBC counts and spleen and liver size are normal
• T cell development and Pi3k-Akt signaling in thymic T cells are normal
increased hematocrit ( J:227340 )
• hematocrit is significantly increased, esp. in female mice
decreased mean corpuscular hemoglobin concentration ( J:227340 )
• MCHC is significantly decreased, esp. in female mice
increased mean corpuscular volume ( J:227340 )
• MCV is significantly increased, esp. in female mice

cellular
abnormal cell physiology ( J:227340 )
• primary MEFs derived from E13.5 embryos exhibit decreased Akt activation upon stimulation with HGF; in contrast, FBS- or EGF-induced Akt activation is relatively normal
• in vivo, basal Akt activity is not significantly altered in stomach, liver or muscle tissues
decreased fibroblast cell migration ( J:227340 )
• primary MEFs derived from E13.5 embryos exhibit reduced HGF-induced transmembrane migration and decreased movement in a wound-healing assay as well as markedly decreased HGF-triggered invasion in a matrigel assay
• in contrast, EGF-induced migration and invasion are normal




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Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory