Phenotypes associated with this allele

• Allele Symbol: Casc3^{tm1(KOMP)Vlcg}
• Allele Name: targeted mutation 1, Velocigene
• Allele ID: MGI:4452843
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Casc3^tm1(KOMP)Vlcg/Casc3^tm1(KOMP)Vlcg	involves: C57BL/6J * C57BL/6NTac	MGI:5888879
ht2	Casc3^tm1(KOMP)Vlcg/Casc3^+	involves: C57BL/6J * C57BL/6NTac	MGI:5888880
ht3	Casc3^Gt(RRU345)Byg/Casc3^tm1(KOMP)Vlcg	involves: 129P2/OlaHsd * C57BL/6J * C57BL/6NTac	MGI:5888881

Genotype
MGI:5888879

hm1

• Allelic Composition: Casc3^{tm1(KOMP)Vlcg}/Casc3^{tm1(KOMP)Vlcg}
• Genetic Background: involves: C57BL/6J * C57BL/6NTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:240595 )

• all homozygous mutant embryos die between E10.5 and E11.5

embryo

embryonic growth retardation ( J:240595 )

• at E10.5, homozygous mutant embryos exhibit developmental delay

abnormal embryo morphology ( J:240595 )

• at E10.5, homozygous mutant embryos appear dysmorphic relative to wild-type embryos

decreased embryo size ( J:240595 )

• at E10.5, homozygous mutant embryos are markedly smaller than wild-type embryos

growth/size/body

embryonic growth retardation ( J:240595 )

• at E10.5, homozygous mutant embryos exhibit developmental delay

decreased embryo size ( J:240595 )

• at E10.5, homozygous mutant embryos are markedly smaller than wild-type embryos

Genotype
MGI:5888880

ht2

• Allelic Composition: Casc3^{tm1(KOMP)Vlcg}/Casc3⁺
• Genetic Background: involves: C57BL/6J * C57BL/6NTac

normal phenotype

no abnormal phenotype detected ( J:240595 )

• heterozygous mutant embryos exhibit normal size and morphology at E10.5; no overt reduction in brain size is noted at E11.5 or E12.5

Genotype
MGI:5888881

ht3

• Allelic Composition: Casc3^{Gt(RRU345)Byg}/Casc3^{tm1(KOMP)Vlcg}
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J * C57BL/6NTac

growth/size/body

decreased fetal size ( J:240595 )

• body size is significantly reduced at E13.5 and E14.5

fetal growth retardation ( J:240595 )

• at E13.5 and E14.5, compound heterozygotes are developmentally delayed by 1 day relative to wild-type controls, as noted by eye pigmentation and limb development

limbs/digits/tail

delayed limb development ( J:240595 )

• at E14.5, limb development is delayed relative to that in wild-type controls

nervous system

nervous system phenotype ( J:240595 )

N • although cortical thickness, number of TBR2+ intermediate neural progenitors, and thickness of the TUJ1+ neuronal layer are reduced at E14.5, these differences are largely due to developmental delay, as E14.5 values are similar to those in E13.5 wild-type brains

N • no significant apoptosis is noted in the cortex at E14.5