Phenotypes associated with this allele

• Allele Symbol: Zfyve16^{tm2a(KOMP)Wtsi}
• Allele Name: targeted mutation 2a, Wellcome Trust Sanger Institute
• Allele ID: MGI:4451610
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Zfyve16^tm2a(KOMP)Wtsi/Zfyve16^tm2a(KOMP)Wtsi	C57BL/6N-Zfyve16^tm2a(KOMP)Wtsi	MGI:6513987

Genotype
MGI:6513987

hm1

• Allelic Composition: Zfyve16^{tm2a(KOMP)Wtsi}/Zfyve16^{tm2a(KOMP)Wtsi}
• Genetic Background: C57BL/6N-Zfyve16^{tm2a(KOMP)Wtsi}
• Cell Lines: EPD0514_4_C01

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

decreased B cell proliferation ( J:302014 )

• the number of splenic B cells in the G0 phase is significantly higher whereas the number of splenic B cells in the G1 phase is significantly lower than that in wild-type controls

• in a B-lymphoid cell colony formation assay, both the size and number of B cell colonies is significantly reduced relative to that in wild-type controls

• however, no differences in apoptosis of pre-B cells (from bone marrow) or B cells (from spleen and peripheral blood) are observed

increased common lymphocyte progenitor cell number ( J:302014 )

• the percentage of common lymphoid progenitor cells (CLP, Lin^-c-kit^loCD127⁺) is significantly increased in the bone marrow

• however, bone marrow cell number is normal and no changes in the percentage of bone marrow hematopoietic stem cells (LSK) or myeloid progenitor cells (GMP, CMP, and MEP) are observed

decreased B cell number ( J:302014 )

• at 6 months of age, mice show a decreased percentage of B-lymphoid cells (CD19⁺B220⁺) in peripheral blood relative to wild-type controls

• following a semi-lethal dose of irradiation, the recovery rate of B-lymphoid cells is significantly lower than that in wild-type controls

increased T cell number ( J:302014 )

• at 5 months of age, mice show an increased percentage of T-lymphoid cells (CD3e⁺) in peripheral blood relative to wild-type controls

• following a semi-lethal dose of irradiation, the recovery rate of T-lymphoid cells is significantly higher than that in wild-type controls

immune system

decreased B cell proliferation ( J:302014 )

• the number of splenic B cells in the G0 phase is significantly higher whereas the number of splenic B cells in the G1 phase is significantly lower than that in wild-type controls

• in a B-lymphoid cell colony formation assay, both the size and number of B cell colonies is significantly reduced relative to that in wild-type controls

• however, no differences in apoptosis of pre-B cells (from bone marrow) or B cells (from spleen and peripheral blood) are observed

decreased B cell number ( J:302014 )

• at 6 months of age, mice show a decreased percentage of B-lymphoid cells (CD19⁺B220⁺) in peripheral blood relative to wild-type controls

• following a semi-lethal dose of irradiation, the recovery rate of B-lymphoid cells is significantly lower than that in wild-type controls

increased T cell number ( J:302014 )

• at 5 months of age, mice show an increased percentage of T-lymphoid cells (CD3e⁺) in peripheral blood relative to wild-type controls

• following a semi-lethal dose of irradiation, the recovery rate of T-lymphoid cells is significantly higher than that in wild-type controls

cellular

decreased B cell proliferation ( J:302014 )

• the number of splenic B cells in the G0 phase is significantly higher whereas the number of splenic B cells in the G1 phase is significantly lower than that in wild-type controls

• in a B-lymphoid cell colony formation assay, both the size and number of B cell colonies is significantly reduced relative to that in wild-type controls

• however, no differences in apoptosis of pre-B cells (from bone marrow) or B cells (from spleen and peripheral blood) are observed

growth/size/body

growth/size/body region phenotype ( J:302014 )

N • mice are grossly normal with no significant changes in body weight during the 9-month observation period