Phenotypes associated with this allele

• Allele Symbol: Grin2b^tm1.1Jlbr
• Allele Name: targeted mutation 1.1, Jonathan L Brigman
• Allele ID: MGI:4443321
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Grin2b^tm1.1Jlbr/Grin2b^tm1.1Jlbr Neurod6^tm1(cre)Kan/Neurod6^+	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ	MGI:5307061
cn2	Grin2b^tm1.1Jlbr/Grin2b^tm1.1Jlbr Tg(Camk2a-cre)T29-1Stl/0	involves: 129S6/SvEvTac * BALB/c * C57BL/6	MGI:4443330

Genotype
MGI:5307061

cn1

• Allelic Composition: Grin2b^tm1.1Jlbr/Grin2b^tm1.1Jlbr; Neurod6^tm1(cre)Kan/Neurod6⁺
• Genetic Background: involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal suckling behavior ( J:178913 )

• at P0 a decrease in the number of rhythmic mouth suckling movements in response to stimulation with a feeding needle is seen

• lack of milk in the stomach at P0

increased locomotor activity ( J:178913 )

• increased spontaneous locomotion at P15-P21

social withdrawal ( J:178913 )

Genotype
MGI:4443330

cn2

• Allelic Composition: Grin2b^tm1.1Jlbr/Grin2b^tm1.1Jlbr; Tg(Camk2a-cre)T29-1Stl/0
• Genetic Background: involves: 129S6/SvEvTac * BALB/c * C57BL/6

nervous system

decreased dendritic spine density ( J:159387 )

• CA1 pyramidal neurons exhibit lower spine density of dendrites compared to in wild-type mice

abnormal excitatory postsynaptic currents ( J:159387 )

• unlike in wild-type mice, Ro 25-6981 fails to inhibit eEPSC

decreased excitatory postsynaptic current amplitude ( J:159387 )

• NMDAR-eEPSC amplitude is slightly reduced compared to in wild-type mice

short excitatory postsynaptic current decay time ( J:159387 )

• NMDAR-evoked excitatory postsynaptic current (eEPSC) decay time constants are almost twice as fast as in wild-type mice

abnormal excitatory postsynaptic potential ( J:159387 )

• unlike in wild-type mice, no increase in field excitatory postsynaptic potential (fEPSP) is observed under a protocol designed to evoke modest, subsaturating long term potentiation

• under a protocol designed to produce saturating long term potentiation, mice fail to exhibit a decrease in fEPSP during the first time point compared with similarly treated wild-type mice

• under a long term depression protocol, fEPSP slopes fail to decrease as in similarly treated wild-type mice

• under a long term depression protocol with DL-AP-5 co-application with glutamate transporter inhibitor L-trans-pyrrolidine-2,4-dicarboxylic acid (tPDC), mice exhibit an increase in the fEPSC slope unlike in wild-type mice and an AP-5 washout with another round of stimulation does not produce a lasting decrease in fEPSP slope as observed in similarly treated wild-type mice

reduced long-term potentiation ( J:159387 )

• unlike in wild-type mice, no increase in field excitatory postsynaptic potential (fEPSP) is observed under a protocol designed to evoke modest, subsaturating long term potentiation

• under a protocol designed to produce saturating long term potentiation, mice fail to exhibit a decrease in fEPSP during the first time point compared with similarly treated wild-type mice

absent long-term depression ( J:159387 )

• application of the glutamate transporter inhibitor L-trans-pyrrolidine-2,4-dicarboxylic acid (tPDC) does not produce long term depression unlike in wild-type mice

reduced long-term depression ( J:159387 )

• under a long term depression protocol, fEPSP slopes fail to decrease as in similarly treated wild-type mice

• under a long term depression protocol with DL-AP-5 co-application with glutamate transporter inhibitor L-trans-pyrrolidine-2,4-dicarboxylic acid (tPDC), mice exhibit an increase in the fEPSC slope unlike in wild-type mice and an AP-5 washout with another round of stimulation does not produce a lasting decrease in fEPSP slope as observed in similarly treated wild-type mice

behavior/neurological

abnormal learning/memory/conditioning ( J:159387 )

• in a T maze, mice exhibit fewer spontaneous alternations compared with wild-type mice

abnormal associative learning ( J:159387 )

• mice exhibit less freezing during retrieval of a trace-conditioned fear memory compared with similarly treated wild-type mice

• however, mice exhibit normal delay-conditioned fear memory and freezing during conditioning of before tone presentation

abnormal spatial learning ( J:159387 )

• in a hidden platform Morris water maze, mice exhibit a longer latency in finding a hidden platform and spend less time in a platform quadrant compared with wild-type mice

• however, mice exhibit normal performance during visible platform training

increased thigmotaxis ( J:159387 )

• in a hidden platform Morris water maze but not when the platform is visible

impaired swimming ( J:159387 )

• in a hidden platform Morris water maze, mice swim slower than wild-type mice

• however, swimming is normal when the platform is visible

homeostasis/metabolism

decreased physiological sensitivity to xenobiotic ( J:159387 )

• unlike in wild-type mice, Ro 25-6981 fails to inhibit evoked excitatory postsynaptic current (eEPSC)

• under a long term depression protocol with DL-AP-5 co-application with glutamate transporter inhibitor L-trans-pyrrolidine-2,4-dicarboxylic acid (tPDC), mice exhibit an increase in the fEPSC slope unlike in wild-type mice and an AP-5 washout with another round of stimulation does not produce a lasting decrease in fEPSP slope as observed in similarly treated wild-type mice

• application of tPDC does not produce long term depression unlike in wild-type mice

• however, DL-AP-5 inhibits eEPSP as in wild-type mice