Phenotypes associated with this allele

• Allele Symbol: Dapk1^tm1Akc
• Allele Name: targeted mutation 1, Adi Kimchi
• Allele ID: MGI:4440532
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Dapk1^tm1Akc/Dapk1^tm1Akc	B6.129X1-Dapk1^tm1Akc	MGI:4440535
cx2	Dapk1^tm1Akc/Dapk1^tm1Akc Tg(CAG-EGFP/Map1lc3b)53Nmz/0	involves: 129X1/SvJ * C57BL/6J * C57BL/6NCrj	MGI:4440536

Genotype
MGI:4440535

hm1

• Allelic Composition: Dapk1^tm1Akc/Dapk1^tm1Akc
• Genetic Background: B6.129X1-Dapk1^tm1Akc

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

decreased sensitivity to induced cell death ( J:158109 )

• attenuated cell death in the response of embryonic fibroblasts (MEFs) to various ER stress inducers, including brefeldin A, thapsigargin and tunicamycin

• attenuated caspase-3-like activity in MEFs

• attenuated accumulation of the active form of caspase-3 in MEFs

• decreased renal tubule apoptosis (shown by TUNEL staining) caused by systemic tunicamycin injection

• normal death responses of the MEFs to UV light, adriamycin and staurosporine

decreased renal tubule apoptosis ( J:158109 )

• decreased renal tubule apoptosis (shown by TUNEL staining) caused by systemic tunicamycin injection

renal/urinary system

decreased renal tubule apoptosis ( J:158109 )

• decreased renal tubule apoptosis (shown by TUNEL staining) caused by systemic tunicamycin injection

abnormal renal tubule morphology ( J:158109 )

• decreased renal tubule apoptosis (shown by TUNEL staining) caused by systemic tunicamycin injection

• more limited tubular damage in the kidney

• small vacuoles in cytoplasm of tubular cells in some areas

• the cellular and tubular architecture is preserved

Genotype
MGI:4440536

cx2

• Allelic Composition: Dapk1^tm1Akc/Dapk1^tm1Akc; Tg(CAG-EGFP/Map1lc3b)53Nmz/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6J * C57BL/6NCrj

cellular

abnormal cell morphology ( J:158109 )

• inhibited GFP-LC3 dots accumulation in embryonic fibroblasts (MEFs)

• reduced number of GFP-LC3 dots/mm2 in MEFs treated with thapsigargin for 24 h