Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgif2tm1Dwot mutation
(0 available);
any
Tgif2 mutation
(40 available)
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normal phenotype
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• viable with no gross defects
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nervous system
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• reduced Gli3 levels partially rescues ventral forebrain morphology observed in double mutants with less disorganization and partially separated cephalic folds
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• in half of mice at E18.5
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embryo
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• partially separated cephalic folds
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craniofacial
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• partially rescued nasal field separation defect
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growth/size/body
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• partially rescued nasal field separation defect
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respiratory system
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• partially rescued nasal field separation defect
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taste/olfaction
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• partially rescued nasal field separation defect
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nervous system
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• reduced Nodal levels partially rescues ventral neurepithelium morphogenesis observed in double mutants with disorganized neuroepithelium
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• reduced Nodal levels partially rescues ventral forebrain morphology observed in double mutants with less disorganization and larger size
• however, forebrain cell proliferation is restored
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• in mice that survive to E18.5
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embryo
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• expression analysis suggests there are some mild defects in left right patterning
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• severe anterior truncation in a small proportion of mice
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• in 2 embryos with severe anterior truncation
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• reduced Nodal levels partially rescues ventral neurepithelium morphogenesis observed in double mutants with disorganized neuroepithelium
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growth/size/body
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• in 2 embryos with severe anterior truncation
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cardiovascular system
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N |
• unlike in double null mice wild-type for Nodal, heart looping morphogenesis is normal
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mortality/aging
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• most mice do not survive beyond E11.0
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nervous system
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• without any indication of ventral morphology
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• midbrain neural tube fails to close at E9.25
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• at E10.0
• however, mice exhibit normal forebrain size and morphology at E9.0
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• single thickened layer of surface ectoderm in the ventral forebrain and the nasal field has not separated by E10.0
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• at E9.0 and more so at E10.0
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embryo
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• bilateral expression of normally unilateral genes is seen in the lateral plate mesoderm at E8.5
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• fused ventral lips at E8.25
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• without any indication of ventral morphology
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• midbrain neural tube fails to close at E9.25
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• immunofluorescence and immunohistochemical studies suggest AP axis has been specified, and the primitive streak has been induced, but fails to elongate
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growth/size/body
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• in one of the two surviving embryos at E12.5
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respiratory system
taste/olfaction
vision/eye
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• single small pigmented eye field vesicle
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• in the two surviving embryos at E12.5
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cardiovascular system
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• in 24% the heart loop has extended dorsoventrally
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• 35% have a reversal (right to left) of looping direction
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• in about a quarter of embryos the heart extends without looping
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craniofacial
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• in one of the two surviving embryos at E12.5
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cellular
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• in the forebrain at E10.0
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Nodaltm1Rob mutation
(1 available);
any
Nodal mutation
(41 available)
Tgif1tm1Dwot mutation
(0 available);
any
Tgif1 mutation
(52 available)
Tgif2tm1Dwot mutation
(0 available);
any
Tgif2 mutation
(40 available)
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embryo
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N |
• unlike in double null mice wild-type for Nodal, the embryonic cavity has formed by E8.5 and a distinct AP axis is present
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgif1tm1Dwot mutation
(0 available);
any
Tgif1 mutation
(52 available)
Tgif2tm1Dwot mutation
(0 available);
any
Tgif2 mutation
(40 available)
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reproductive system
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• often show reduced fertility
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growth/size/body
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• tend to be smaller than their littermates
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embryo
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• a small proportion of embryos at E6.5 have a rounder appearance suggesting decreased elongation of the proximodistal axis
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgif1tm1Dwot mutation
(0 available);
any
Tgif1 mutation
(52 available)
Tgif2tm1Dwot mutation
(0 available);
any
Tgif2 mutation
(40 available)
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mortality/aging
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• unable to identify any embryos after E9.5
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growth/size/body
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• significantly smaller at E7.5 and E8.5
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embryo
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• at E7.5 formation of the germ layers appears impaired
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• formation or morphogenesis of germ layers is impaired resulting in an endodermal layer surrounding a disorganized mass of cells
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• lack of epithelial morphology, without a transition to mesodermal fate based upon E-cadherin or N-cadherin expression
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• at 6.5 rounder appearance of the embyros suggests decreased elongation of the proximodistal axis
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• unable to identify a clear AP axis, even at 8.5 dpc
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• at E6.5 cells within the epiblast are less well organized
• at E7.5 and later cells of the epiblast fail to display the characteristic columnar epithelial morphology and the embryo has not formed a distinct embryonic cavity
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• at E7.5 proliferation rate is decreased in the epiblast
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• immunofluorescence and immunohistochemical studies suggest AP axis has been specified, and the primitive streak has been induced, but fails to elongate
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cellular
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• at E7.5 proliferation rate is decreased in the epiblast
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tgif1tm1Dwot mutation
(0 available);
any
Tgif1 mutation
(52 available)
Tgif2tm1Dwot mutation
(0 available);
any
Tgif2 mutation
(40 available)
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embryo
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• a small proportion of embryos at E6.5 have a rounder appearance suggesting decreased elongation of the proximodistal axis
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growth/size/body
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• tend to be smaller than their littermates
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reproductive system
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• often show reduced fertility
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Analysis Tools